Madorran Eneko, Ambrož Miha, Knez Jure, Sobočan Monika
Faculty of Medicine, Institute of Anatomy, Histology and Embryology, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia.
Faculty of Medicine, Institute of Translational and Clinical Research, University of Maribor, Taborska ulica 8, 2000 Maribor, Slovenia.
Int J Mol Sci. 2024 Dec 30;26(1):220. doi: 10.3390/ijms26010220.
Over the past century, numerous methods for assessing cell viability have been developed, and there are many different ways to categorize these methods accordingly. We have chosen to use the Organisation for Economic Co-operation and Development (OECD) classification due to its regulatory importance. The OECD categorizes these methods into four groups: non-invasive cell structure damage, invasive cell structure damage, cell growth, and cellular metabolism. Despite the variety of cell viability methods available, they can all be categorized within these four groups, except for two novel methods based on the cell membrane potential, which we added to the list. Each method operates on different principles and has its own advantages and disadvantages, making it essential for researchers to choose the method that best fits their experimental design. This review aims to assist researchers in making this decision by describing these methods regarding their potential use and providing direct references to the cell viability assessment methods. Additionally, we use the OECD classification to facilitate potential regulatory use and to highlight the need for adding a new category to their list.
在过去的一个世纪里，已经开发出了许多评估细胞活力的方法，并且有许多不同的方式对这些方法进行相应分类。由于其在监管方面的重要性，我们选择使用经济合作与发展组织（OECD）的分类方法。OECD将这些方法分为四类：非侵入性细胞结构损伤、侵入性细胞结构损伤、细胞生长和细胞代谢。尽管有各种各样的细胞活力评估方法，但除了我们添加到列表中的两种基于细胞膜电位的新方法外，它们都可以归类在这四类之中。每种方法的操作原理不同，都有其自身的优缺点，这使得研究人员必须选择最适合其实验设计的方法。本综述旨在通过描述这些方法的潜在用途并提供细胞活力评估方法的直接参考文献，来帮助研究人员做出这一决策。此外，我们使用OECD分类方法以促进其在潜在监管方面的应用，并强调有必要在其列表中增加一个新类别。
