Díez-Sainz Ester, Milagro Fermín I, Aranaz Paula, Riezu-Boj José I, Batrow Pierre-Louis, Contu Laura, Gautier Nadine, Amri Ez-Zoubir, Mothe-Satney Isabelle, Lorente-Cebrián Silvia
Department of Nutrition, Food Science and Physiology, and Center for Nutrition Research, Faculty of Pharmacy and Nutrition, University of Navarra, 31008 Pamplona, Spain.
Navarra Institute for Health Research (IdiSNA), 31008 Pamplona, Spain.
Int J Mol Sci. 2024 Dec 31;26(1):276. doi: 10.3390/ijms26010276.
MicroRNAs play a pivotal role in the regulation of adipose tissue function and have emerged as promising therapeutic candidates for the management of obesity and associated comorbidities. Among them, miR-1 could be a potential biomarker for metabolic diseases and contribute to metabolic homeostasis. However, thorough research is required to fully elucidate the impact of miR-1 on human adipocyte thermogenesis and metabolism. This study aimed to explore the effect of miR-1 on human adipocyte browning, a process whose activation has been linked to obesity protection and counteraction. Human multipotent adipose-derived stem cells, hMADS cells, were differentiated into white and brown-like adipocytes and transfected with miR-1 mimics for gene expression and western blotting analyses. miR-1 inhibited the expression of its previously validated target / and modulated the expression profile of key genes involved in thermogenesis and adipocyte browning (increased at mRNA and protein level, increased , decreased ), adipocyte differentiation and metabolism (decreased , , , , , ), as well as genes related to the cytoskeleton (decreased ) and extracellular matrix (decreased ). These findings suggest that miR-1 can modulate the expression of adipocyte human genes associated with thermogenesis and metabolism, which could hold value for eventual therapeutic potential in obesity.
微小RNA在脂肪组织功能的调节中起关键作用，并已成为治疗肥胖症及相关合并症的有前景的候选药物。其中，miR-1可能是代谢性疾病的潜在生物标志物，并有助于代谢稳态。然而，需要进行深入研究以充分阐明miR-1对人类脂肪细胞产热和代谢的影响。本研究旨在探讨miR-1对人类脂肪细胞褐变的影响，这一过程的激活与肥胖保护和对抗有关。人多能脂肪来源干细胞（hMADS细胞）被分化为白色和棕色样脂肪细胞，并用miR-1模拟物转染以进行基因表达和蛋白质印迹分析。miR-1抑制其先前验证的靶标的表达，并调节参与产热和脂肪细胞褐变（在mRNA和蛋白质水平上增加，增加，减少）、脂肪细胞分化和代谢（减少，，，，，）以及与细胞骨架（减少）和细胞外基质（减少）相关基因的表达谱。这些发现表明，miR-1可以调节与产热和代谢相关的人类脂肪细胞基因的表达，这对于肥胖症最终的治疗潜力可能具有价值。
