Song Yingjie, Yang Lan, Han Yao, Li Wenjun, Wei Tong, Gao Yamin, Hu Qiang, Li Hao, Sun Yansong
State Key Laboratory of Pathogen and Biosecurity, Academy of Military Medical Sciences, Beijing 100071, China.
Int J Mol Sci. 2025 Jan 2;26(1):339. doi: 10.3390/ijms26010339.
RAD52 plays crucial roles in several aspects of mammalian cells, including DNA double-strand breaks repair, viral infection, cancer development, and antibody class switching. To comprehensively elucidate the role of RAD52 in maintaining genome stability and uncover additional functions of RAD52 in mammals, we performed the transcriptomics and proteomics analysis of the liver of knockout mice. Transcriptomics analysis reveals overexpression of mitochondrial genes in the liver of knockout (RAD52KO) mice. Proteomics analysis of RAD52KO mice shows that damage recognition proteins Cul4b and Rad23a in the process of nucleotide excision repair pathway are overexpressed. Furthermore, gene ontology and KEGG enrichment analysis (accessed on 20 November 2024) from integrated omics shows that differentially expressed genes are significantly enriched in pathways related to mitochondrial oxidative phosphorylation and nucleotide metabolism in the liver of RAD52KO mice. In addition, mRNA and protein levels of Bhmt1b are elevated in the liver of RAD52KO mice. Taken together, this study provides valuable insights into the function and mechanism of RAD52.
RAD52在哺乳动物细胞的多个方面发挥着关键作用，包括DNA双链断裂修复、病毒感染、癌症发展和抗体类别转换。为了全面阐明RAD52在维持基因组稳定性中的作用，并揭示RAD52在哺乳动物中的其他功能，我们对敲除小鼠的肝脏进行了转录组学和蛋白质组学分析。转录组学分析显示，敲除（RAD52KO）小鼠肝脏中的线粒体基因过表达。对RAD52KO小鼠的蛋白质组学分析表明，核苷酸切除修复途径过程中的损伤识别蛋白Cul4b和Rad23a过表达。此外，综合组学的基因本体论和KEGG富集分析（于2024年11月20日获取）表明，RAD52KO小鼠肝脏中差异表达的基因在与线粒体氧化磷酸化和核苷酸代谢相关的途径中显著富集。此外，RAD52KO小鼠肝脏中Bhmt1b的mRNA和蛋白质水平升高。综上所述，本研究为RAD52的功能和机制提供了有价值的见解。
