使用多重数字PCR技术成功检测和定量谷物及食品样本中转基因事件的注意事项
Considerations for the Successful Detection and Quantification of Genetically Modified Events in Grain and Food Samples Using Multiplex Digital PCR.
作者信息
Demeke Tigst
机构信息
Grain Research Laboratory, Canadian Grain Commission, Winnipeg, MB R3P1N1, Canada.
出版信息
Foods. 2024 Dec 31;14(1):75. doi: 10.3390/foods14010075.
Abstract
The number of genetically modified (GMO) events for canola, corn, and soybean is steadily increasing. Some countries, including those in the EU, have regulatory requirements for the approval and use of plant ingredients containing GMOs. Multiplex digital PCR (dPCR) has been used for the simultaneous detection and quantification of various GMO events. This review covers the various factors to consider for multiplex digital PCR detection and the quantification of GMO events. DNA quality, quantity, and the presence of inhibitors are important factors to consider. Some dPCR instruments allow the use of multiple fluorescent dyes, which facilitates the setup of multiplex dPCR assays. This review focuses on the optimization of multiplex dPCR and describes the multiplex dPCR assays that have been reported for GMO detection.
摘要
油菜、玉米和大豆的转基因事件数量正在稳步增加。包括欧盟国家在内的一些国家对含有转基因生物的植物成分的审批和使用有监管要求。多重数字PCR（dPCR）已用于同时检测和定量各种转基因事件。本综述涵盖了多重数字PCR检测和转基因事件定量时需要考虑的各种因素。DNA质量、数量和抑制剂的存在是需要考虑的重要因素。一些dPCR仪器允许使用多种荧光染料，这有助于多重dPCR检测的设置。本综述重点关注多重dPCR的优化，并描述了已报道的用于转基因生物检测的多重dPCR检测方法。
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