Abreu Danilo José Machado de, Nadaleti Denis Henrique Silva, Andrade Rafaela Pereira, Santos Tamara Leite Dos, Tavares Dérica Gonçalves, Botelho Cesar Elias, Resende Mário Lúcio Vilela de, Duarte Whasley Ferreira
Instituto Nacional de Ciência e Tecnologia do Café (INCT), Lavras CEP 37203-202, MG, Brazil.
Biology Department, Federal University of Lavras (UFLA), Lavras CEP 37203-202, MG, Brazil.
Foods. 2025 Jan 3;14(1):111. doi: 10.3390/foods14010111.
One strategy for adding unique characteristics and flavors to improve coffee quality is the selection of starter microorganisms. Here, we aimed to evaluate the effect of LNFCA11 and B10 as starter cultures on the quality of four different wet-fermented coffee varieties. Microbiological, molecular, and chemical analyses were carried out to identify yeast, bacteria, volatile compounds, carbohydrates and bioactive compounds in coffee. Sensory analysis was performed by Q-graders certified in coffee. Starter yeasts affected bioactive and volatile compounds as well as sensory descriptors in the coffee varieties. CA11 allowed a higher content of trigonelline and chlorogenic acid in MGS Paraíso 2 (P2) and Catuai Amarelo IAC62 (CA62) varieties. B10 fermentation resulted in higher chlorogenic acid only on the P2 cultivar and MGS Catucaí Pioneira (CP). In addition, 5-methyl-2-furfuryl alcohol and n-hexadecanoic acid were produced exclusively by yeast fermentation compared to spontaneous fermentation. The coffee cultivars P2 presented more complex sensory descriptors and the attributes of aroma, acidity, and balance when fermented with CA11. Sensory descriptors such as lemongrass, citrus, and lemon with honey were related to B10. Starter cultures allowed the coffees to be classified as specialty coffees. The fermentation showed that the choice of starter yeast depends on the desired sensory descriptors in the final product.
一种通过添加独特特性和风味来提高咖啡品质的策略是选择起始微生物。在此,我们旨在评估LNFCA11和B10作为起始培养物对四种不同湿法发酵咖啡品种品质的影响。进行了微生物学、分子学和化学分析,以鉴定咖啡中的酵母、细菌、挥发性化合物、碳水化合物和生物活性化合物。由咖啡认证的Q分级师进行感官分析。起始酵母影响了咖啡品种中的生物活性和挥发性化合物以及感官描述符。CA11使MGS帕拉伊索2(P2)和黄卡杜艾IAC62(CA62)品种中的葫芦巴碱和绿原酸含量更高。B10发酵仅在P2品种和MGS卡图凯先锋(CP)上产生了更高的绿原酸。此外,与自然发酵相比,5-甲基-2-糠醇和正十六烷酸仅由酵母发酵产生。用CA11发酵时,咖啡品种P2呈现出更复杂的感官描述符以及香气、酸度和平衡的属性。与B10相关的感官描述符如柠檬草、柑橘和带蜂蜜的柠檬。起始培养物使这些咖啡被归类为特种咖啡。发酵表明,起始酵母的选择取决于最终产品所需的感官描述符。