Niu Yujie, Wang Yongda, Li Lu, Zhang Xiyu, Liu Ting
Department of Physics, Northeast Forestry University, Harbin 150040, China.
School of Instrumentation Science and Engineering, Harbin Institute of Technology, Harbin 150001, China.
Sensors (Basel). 2024 Dec 31;25(1):186. doi: 10.3390/s25010186.
In this study, an approach has been proposed in response to the urgent need for a sensitive and stable method for glucose detection at low concentrations. Platinum octaethylporphyrin (PtOEP) was chosen as the probe and embedded into the matrix material to yield a glucose-sensing film, i.e., Pt/TE-MTS, through a sol-gel process. The optical parameter (OP) was defined as the ratio of phosphorescence in the absence and presence of glucose, and the relationship between OP and glucose concentration (GC) was established in a theoretical way based on the Stern-Volmer equation and further obtained by photoluminescence measurement. OP exhibited a linear relationship with GC in a range of 0-720 μM. The time required by the photoluminescence of the film to reach equilibrium was measured to ensure the completion of the reaction, and it was found that the equilibrium time decreased as the GC increased. The photobleaching behavior and stabilization of the film were monitored, and the result showed that the film exhibited excellent resistance to photobleaching and was quite stable in an aqueous solution. Additionally, a LabVIEW-based GC-detection system was developed to achieve the practical application of the sensing film. In summary, the Pt/TE-MTS film exhibited high sensitivity in detecting the GC with excellent reproducibility, which is of high value in applications.
在本研究中，针对低浓度葡萄糖检测急需一种灵敏且稳定的方法，提出了一种方法。选择铂八乙基卟啉（PtOEP）作为探针，并通过溶胶 - 凝胶法将其嵌入基质材料中，以制备葡萄糖传感膜，即Pt/TE-MTS。将光学参数（OP）定义为葡萄糖存在与不存在时磷光的比值，并基于斯特恩 - 沃尔默方程从理论上建立OP与葡萄糖浓度（GC）之间的关系，进而通过光致发光测量获得。在0 - 720 μM范围内，OP与GC呈现线性关系。测量了膜的光致发光达到平衡所需的时间以确保反应完成，发现平衡时间随GC增加而减少。监测了膜的光漂白行为和稳定性，结果表明该膜表现出优异的抗光漂白性能，并且在水溶液中相当稳定。此外，开发了基于LabVIEW的GC检测系统以实现传感膜的实际应用。总之，Pt/TE-MTS膜在检测GC时表现出高灵敏度和出色的重现性，具有很高的应用价值。
