Lv Miao, Song Xiaoxiao, Wang Weitao, Li Jiale, Chen Jiewen, Huang Xiaolan, Su Li, Gu Lian
School of Public Health, Guangxi Medical University, Nanning, Guangxi, China.
Guangxi University of Chinese Medicine, Nanning, Guangxi, China.
Mol Neurobiol. 2025 May;62(5):6397-6409. doi: 10.1007/s12035-024-04646-y. Epub 2025 Jan 11.
Dysregulation of long non-coding RNAs (lncRNAs) is implicated in the pathophysiology of ischemic stroke (IS). However, the molecular mechanism of the lncRNA SERPINB9P1 in IS remains unclear. Our study aimed to explore the role and molecular mechanism of the lncRNA SERPINB9P1 in IS. This study revealed downregulation of the lncRNA SERPINB9P1 in the peripheral blood of IS patients, which was corroborated by the GSE140275 dataset. Furthermore, high lncRNA SERPINB9P1 expression was associated with lower National Institutes of Health Stroke Scale (NIHSS) scores and favorable outcome. Clinically, lncRNA SERPINB9P1 expression was correlated with inflammation and coagulation parameters in IS patients. Furthermore, lncRNA SERPINB9P1 silencing inhibited cell viability, induced apoptosis and inflammatory response under oxygen-glucose deprivation/reperfusion ; however, these effects were reversed upon its overexpression. Additionally, Chromatin Isolation by RNA Purification and mass spectrometry (CHIRP-MS) and western blot confirmed that the lncRNA SERPINB9P1 was involved in the pathological process of IS through binding to heat shock protein 2 (HSPA2). HSPA2 was upregulated in IS patients, and its protein interaction network was significantly enriched in IS-related pathways. In conclusion, the lncRNA SERPINB9P1 may ameliorate neurological injury in IS patients by interacting with the HSPA2 protein and engaging in IS-related pathways, providing new insights into treatment strategies for IS.
长链非编码RNA（lncRNAs）的失调与缺血性中风（IS）的病理生理学有关。然而，lncRNA SERPINB9P1在IS中的分子机制仍不清楚。我们的研究旨在探讨lncRNA SERPINB9P1在IS中的作用和分子机制。本研究揭示了IS患者外周血中lncRNA SERPINB9P1的下调，这一点在GSE140275数据集中得到了证实。此外，lncRNA SERPINB9P1高表达与较低的美国国立卫生研究院卒中量表（NIHSS）评分及良好预后相关。临床上，lncRNA SERPINB9P1表达与IS患者的炎症和凝血参数相关。此外，lncRNA SERPINB9P1沉默抑制了氧糖剥夺/再灌注条件下的细胞活力，诱导了细胞凋亡和炎症反应；然而，其过表达可逆转这些效应。此外，通过RNA纯化和质谱进行的染色质分离（CHIRP-MS）及蛋白质免疫印迹证实，lncRNA SERPINB9P1通过与热休克蛋白2（HSPA2）结合参与了IS的病理过程。HSPA2在IS患者中上调，其蛋白质相互作用网络在IS相关通路中显著富集。总之，lncRNA SERPINB9P1可能通过与HSPA2蛋白相互作用并参与IS相关通路来改善IS患者的神经损伤，为IS的治疗策略提供了新的见解。
