Hayes T K, Keeley L L
Gen Comp Endocrinol. 1985 Feb;57(2):246-56. doi: 10.1016/0016-6480(85)90269-2.
The ability of the fat body to respond to the hypertrehalosemic action of corpora cardiaca-allata (CC + CA) extracts was measured in vitro with tissue from adult, male Blaberus discoidalis cockroaches. Only trehalose increased in the hemolymph when gland extracts were injected in vivo; likewise, only trehalose levels responded when fat bodies were exposed to gland extracts in vitro. Although fat bodies from 0-day-old animals gave the least response to gland extracts, all animals 5 or more days old showed elevated responses. A linear dose-response occurred between 0.02 and 0.08 CC + CA pair. Fat bodies did not show an in vitro, hypertrehalosemic response to insulin, glucagon, proctolin, dopamine, epinephrine, norepinephrine, serotonin, octopamine, or tyramine. A strong hypertrehalosemic response was found to both synthetic adipokinetic hormone and red-pigment concentrating hormone; however, reversed-phase, thin-layer chromatography of CC + CA extracts and adipokinetic hormone indicated that the hypertrehalosemic effect and the adipokinetic hormone were separate. This in vitro system comprises a relatively rapid, simple, and accurate bioassay for the hypertrehalosemic neurohormone of insects.
利用成年雄性黄斑黑蝗的组织在体外测定了脂肪体对心侧体-咽侧体（CC + CA）提取物高海藻糖血症作用的反应能力。当在体内注射腺体提取物时，血淋巴中只有海藻糖增加；同样，当脂肪体在体外暴露于腺体提取物时，只有海藻糖水平有反应。虽然0日龄动物的脂肪体对腺体提取物的反应最小，但所有5日龄及以上的动物反应增强。在0.02至0.08对CC + CA之间出现线性剂量反应。脂肪体对胰岛素、胰高血糖素、促肠动素、多巴胺、肾上腺素、去甲肾上腺素、5-羟色胺、章鱼胺或酪胺在体外不表现出海藻糖血症反应。对合成的促脂肪动素和红色素浓缩激素均发现有强烈的高海藻糖血症反应；然而，CC + CA提取物和促脂肪动素的反相薄层色谱表明，高海藻糖血症效应和促脂肪动素是分开的。这个体外系统为昆虫的高海藻糖血症神经激素提供了一种相对快速、简单且准确的生物测定方法。
