Anvekar Mitaj Pandharinath, Virupaxi Shruti Girish, Yavagal Chandrashekar, Kulkarni Sadanand, Pai Ramya, Patil Viplavi Vijaysinh Chavan
Department of Pediatric and Preventive Dentistry, Maratha Mandal's Nathajirao G. Halgekar Institute of Dental Sciences and Research Centre, Belagavi, Karnataka, India.
J Indian Soc Pedod Prev Dent. 2024 Oct 1;42(4):280-285. doi: 10.4103/jisppd.jisppd_258_24. Epub 2025 Jan 11.
The purpose of this study was to evaluate the antimicrobial activity of methylene blue dye with 660-nm diode laser in cavity disinfection and to compare the total bacterial count in dentinal samples preexcavation, postexcavation, and postdisinfection. The study design was experimental in vivo.
Fifteen children aged 5-12 years with 15 primary molars requiring atraumatic restorative treatment (ART) were selected. Dentinal samples were collected from the carious tooth with a sterile spoon excavator three times, i.e., before caries excavation (preexcavation), after hand excavation of caries (postexcavation), and after doing laser photodynamic therapy with methylene blue dye and 660-nm diode laser (postdisinfection) and sent for microbiological analysis. The samples were transported in reduced transport fluid. The total bacterial count was carried out by an experienced microbiologist immediately after reporting.
A significant number of bacteria was present after hand excavation of caries using ART. The bacterial count significantly reduced postlaser disinfection. The total viable bacterial count was reduced to 87.17% with hand excavation of caries, whereas it was reduced to 98.59% postlaser disinfection using methylene blue dye with 660-nm diode laser.
It was seen that ART cannot eliminate all the microorganisms. Methylene blue dye with a 660-nm diode laser was able to reduce the total bacterial count and can be effectively used as an antimicrobial cavity disinfectant.
本研究旨在评估亚甲蓝染料联合660纳米二极管激光在窝洞消毒中的抗菌活性，并比较龋洞预备前、预备后和消毒后的牙本质样本中的细菌总数。本研究设计为体内实验。
选取15名年龄在5至12岁、有15颗需要进行非创伤性修复治疗（ART）的乳牙的儿童。用无菌匙形挖器从龋洞中采集牙本质样本三次，即龋洞预备前（预备前）、手工去除龋坏组织后（预备后）以及用亚甲蓝染料和660纳米二极管激光进行激光光动力治疗后（消毒后），并送去进行微生物分析。样本在运送培养基中运送。报告后，由经验丰富的微生物学家立即进行细菌总数检测。
使用ART手工去除龋坏组织后存在大量细菌。激光消毒后细菌数量显著减少。使用ART手工去除龋坏组织后，活菌总数减少至87.17%，而使用亚甲蓝染料联合660纳米二极管激光进行激光消毒后，活菌总数减少至98.59%。
可见ART不能消除所有微生物。亚甲蓝染料联合660纳米二极管激光能够减少细菌总数，可有效用作抗菌窝洞消毒剂。
