Dubey Ankit Prakashvir, Thalla Arun Kumar
Bioprocesses Engineering Laboratory, Department of Civil Engineering, National Institute of Technology Karnataka, Surathkal 575025, India.
J Hazard Mater. 2025 Apr 5;487:137139. doi: 10.1016/j.jhazmat.2025.137139. Epub 2025 Jan 6.
Plastic pollution, especially microplastics (MPs), is a severe environmental threat. Due to the significant environmental issues posed by plastics, it is critical to use an effective and sustainable degradation technique. The study aimed to isolate and identify Indigenous bacterial strains from landfill leachate (LL) to evaluate its potential for degrading Polypropylene microplastics (PPMPs). The investigation identified two bacterial strains, Pseudomonas aeruginosa, and novel Staphylococcus haemolyticus, through 16S rRNA analysis, capable of decomposing PPMPs. Following a 30-day treatment period, it was noted that Staphylococcus haemolyticus reduced the dry weight of PPMPs by 25.46 % ± 1.35 %, whereas Pseudomonas aeruginosa strain reduced it by 7.01 % ± 0.85 %. Multiple tests, including weight loss, pH, optical density, total biomass yield, and BATH test of the medium, validated the growth of bacterial strains. The biochemical characteristics of the isolated strains were assessed through Biochemical tests. The study also investigated the surface, chemical, and structural changes in treated PPMPs using Scanning electron microscopy (SEM), Energy-Dispersive X-ray spectroscopy (EDS), X-ray diffractometer (XRD), and Ion Chromatography (IC) tests. The Fourier Transform Infrared Spectroscopy (FTIR) study also showed the creation of alcohol, methyl, as well as carbonyl groups due to hydrolysis and oxidation by both bacterial strains. This study implies that the Staphylococcus haemolyticus and Pseudomonas aeruginosa bacterial strains are secure and efficient for PPMP bioremediation.
塑料污染，尤其是微塑料，是一种严重的环境威胁。由于塑料带来的重大环境问题，采用有效且可持续的降解技术至关重要。本研究旨在从垃圾渗滤液中分离并鉴定本土细菌菌株，以评估其降解聚丙烯微塑料的潜力。通过16S rRNA分析，该调查鉴定出两种细菌菌株，即铜绿假单胞菌和新型溶血葡萄球菌，它们能够分解聚丙烯微塑料。经过30天的处理期后，发现溶血葡萄球菌使聚丙烯微塑料的干重减少了25.46%±1.35%，而铜绿假单胞菌菌株使其减少了7.01%±0.85%。包括重量损失、pH值、光密度、总生物量产量以及培养基的BATH测试在内的多项测试，验证了细菌菌株的生长情况。通过生化测试评估了分离菌株的生化特性。该研究还使用扫描电子显微镜（SEM）、能量色散X射线光谱仪（EDS）、X射线衍射仪（XRD）和离子色谱（IC）测试，研究了处理后聚丙烯微塑料的表面、化学和结构变化。傅里叶变换红外光谱（FTIR）研究还表明，由于两种细菌菌株的水解和氧化作用，产生了醇、甲基以及羰基基团。本研究表明，溶血葡萄球菌和铜绿假单胞菌菌株对于聚丙烯微塑料的生物修复是安全且有效的。
