S Ankul Singh, Vellapandian Chitra
Department of Pharmacology, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu 603203, India.
Department of Pharmacology, SRM College of Pharmacy, SRM Institute of Science and Technology, Kattankulathur, Tamil Nadu 603203, India.
Ecotoxicol Environ Saf. 2025 Jan 1;289:117686. doi: 10.1016/j.ecoenv.2025.117686. Epub 2025 Jan 10.
Luffa cylindrica (L.), is a medicinal plant aimed to investigate the efficacy of the alkaloid-rich fraction (ARF) extracted from L. cylindrica. The study employed behavioural analysis of rat using Morris water maze (MWM), biochemical analysis of apoptotic proteins, Immunohistochemistry of caspase 3 protein and network pharmacology approach. The ozone-induced group exhibited complex behavioral changes. Western blot analysis revealed altered expression of SOD2, Caspase 3, and Cytochrome C which play integral roles in oxidative stress, apoptosis, and mitochondrial function. Histopathological analysis of the hippocampus further supported the neuroprotective potential of L. cylindrica, demonstrating a reduction in neuropathological lesions and improved memory processes. Network Pharmacology showed the implication of GSK3β in neuronal damage. ARF showed promise in preventing further neuronal damage. In summary, this comprehensive study sheds light on its potential in neuroprotective applications by in vivo behavioral and molecular analyses. It provides a holistic understanding of the medicinal properties of ARF, encouraging further exploration for potential therapeutic interventions in neurological diseases.
丝瓜（Luffa cylindrica (L.)）是一种药用植物，旨在研究从丝瓜中提取的富含生物碱的部分（ARF）的功效。该研究采用了使用莫里斯水迷宫（MWM）对大鼠进行行为分析、对凋亡蛋白进行生化分析、对caspase 3蛋白进行免疫组织化学分析以及网络药理学方法。臭氧诱导组表现出复杂的行为变化。蛋白质印迹分析显示，在氧化应激、细胞凋亡和线粒体功能中起重要作用的SOD2、Caspase 3和细胞色素C的表达发生了改变。海马体的组织病理学分析进一步支持了丝瓜的神经保护潜力，表明神经病理损伤减少且记忆过程得到改善。网络药理学表明GSK3β与神经元损伤有关。ARF在预防进一步的神经元损伤方面显示出前景。总之，这项全面的研究通过体内行为和分子分析揭示了其在神经保护应用中的潜力。它提供了对ARF药用特性的全面理解，鼓励进一步探索针对神经疾病的潜在治疗干预措施。
