Wang Yupeng, Wang Yi, Cui Jiakai, Wu Chenchen, Yu Bo, Wang Limin
Department of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Department of Microbial Physiological and Metabolic Engineering, State Key Laboratory of Mycology, Institute of Microbiology, Chinese Academy of Sciences, Beijing 100101, China; School of Life Sciences, Yunnan University, Kunming 650500, China.
Trends Biotechnol. 2025 Jan 10. doi: 10.1016/j.tibtech.2024.12.004.
Microbial production of organic acids has been hindered by the poor acid tolerance of microorganisms and the high costs of waste salt reprocessing. The robustness of non-conventional microorganisms in an acidic environment makes it possible to produce organic acids at low pH and greatly simplifies downstream processing. In this review we discuss the environmental adaptability features of non-conventional yeasts, as well as the latest developments in genomic engineering strategies that have facilitated metabolic engineering of these strains. We also use selected examples of three-carbon (C3), C4, and C6 organic acids to illustrate the ongoing efforts and challenges of using non-conventional yeasts for organic acid production. This review provides theoretical guidance for the construction of highly robust organic acid producers.
微生物对酸的耐受性较差以及废盐再处理成本高昂，阻碍了有机酸的微生物生产。非常规微生物在酸性环境中的稳健性使得在低pH值下生产有机酸成为可能，并极大地简化了下游加工过程。在这篇综述中，我们讨论了非常规酵母的环境适应性特征，以及促进这些菌株代谢工程的基因组工程策略的最新进展。我们还使用三碳（C3）、C4和C6有机酸的选定实例来说明使用非常规酵母生产有机酸的持续努力和挑战。这篇综述为构建高度稳健的有机酸生产者提供了理论指导。
