Doszyn Olga, Dulski Tomasz, Zmorzynska Justyna
Laboratory of Developmental Neurobiology, International Institute of Molecular Mechanisms and Machines, 02-247 Warsaw, Poland; Laboratory of Molecular and Cellular Neurobiology, International Institute of Molecular and Cell Biology in Warsaw, 02-109 Warsaw, Poland.
Laboratory of Developmental Neurobiology, International Institute of Molecular Mechanisms and Machines, 02-247 Warsaw, Poland.
STAR Protoc. 2025 Mar 21;6(1):103566. doi: 10.1016/j.xpro.2024.103566. Epub 2025 Jan 11.
Mechanistic target of rapamycin complex 1 (mTorC1) activity plays a crucial role in brain development. Here, we present an approach for rapamycin microinjection into the habenula of larval zebrafish to achieve localized inhibition of the mTorC1 pathway and explore the role of mTorC1 in habenula function. We describe steps for performing microinjections and maintaining zebrafish larvae before and after the procedure. For complete details on the use and execution of this protocol, please refer to Doszyn et al..
雷帕霉素机制性靶标复合物1（mTorC1）的活性在大脑发育中起着至关重要的作用。在此，我们提出一种将雷帕霉素显微注射到斑马鱼幼体缰核中的方法，以实现对mTorC1通路的局部抑制，并探索mTorC1在缰核功能中的作用。我们描述了进行显微注射以及在该操作前后饲养斑马鱼幼体的步骤。有关此方案的使用和执行的完整详细信息，请参考多辛等人的研究。
