He Huihui, Li Ji, Zhuang Jiayi, Huang Jinyi, Meng Yuxuan, Lin Xi, Wei Zhangwen, Zhang Liangliang, Fang Yu, Pan Mei
Strait Institute of Flexible Electronics (SIFE, Future Technologies), Fujian Normal University and Strait Laboratory of Flexible Electronics (SLoFE), Fuzhou 350017, China.
State Key Laboratory of Chemo/Bio-Sensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha, Hunan, 410082, China.
Angew Chem Int Ed Engl. 2025 Feb 24;64(9):e202420912. doi: 10.1002/anie.202420912. Epub 2025 Jan 21.
Immobilizing organic chromophores within the rigid framework of metal-organic frameworks (MOFs) augments fluorescence by effectively curtailing molecular motions. Yet, the substantial interspaces and free volumes inherent to MOFs can undermine photoluminescence efficiency, as they partially constrain intramolecular dynamics. In this study, we achieved optimization of both one- and two-photon excited fluorescence by incorporating linkers into an interpenetrated tetraphenylethene-based MOF (TPE-MOF). This linker installation strategy enables fine-tuning of both crystal packing density and ligand conformations. Strikingly, the desolvated MOFs exhibit remarkable two-photon absorption (TPA) cross-sections, reaching an impressive 8801 GM. Consequently, these materials demonstrate exceptional performance in one- and two-photon excited cellular imaging of HepG2 cells. Our work introduces an innovative approach to enhancing two-photon excited fluorescence (TPEF) and broadens the scope of research into one- and two-photon excited fluorescence (1/2 PEF).
将有机发色团固定在金属有机框架（MOF）的刚性骨架内，通过有效抑制分子运动增强荧光。然而，MOF固有的大量间隙和自由体积会削弱光致发光效率，因为它们部分限制了分子内动力学。在本研究中，我们通过将连接体引入互穿的基于四苯基乙烯的MOF（TPE-MOF）中，实现了单光子和双光子激发荧光的优化。这种连接体安装策略能够对晶体堆积密度和配体构象进行微调。引人注目的是，去溶剂化的MOF表现出显著的双光子吸收（TPA）截面，达到了令人印象深刻的8801 GM。因此，这些材料在HepG2细胞的单光子和双光子激发细胞成像中表现出卓越的性能。我们的工作引入了一种增强双光子激发荧光（TPEF）的创新方法，并拓宽了单光子和双光子激发荧光（1/2 PEF）的研究范围。
