Abdelmaksoud Nourhan M, Abulsoud Ahmed I, Abdelghany Tamer M, Elshaer Shereen Saeid, Samaha Ahmed, Maurice Nadine W, Rizk Sherine Maher, Senousy Mahmoud A
Department of Biochemistry, Faculty of Pharmacy, Heliopolis University, 3 Cairo-Belbeis Desert Road, P.O. Box 3020El Salam,, 11785, Cairo, Egypt.
Department of Biochemistry and Molecular Biology, Faculty of Pharmacy (Boys), Al-Azhar University, Nasr City, Cairo, 11823, Egypt.
J Egypt Natl Canc Inst. 2025 Jan 13;37(1):2. doi: 10.1186/s43046-024-00255-x.
Colorectal cancer (CRC) is a major public health concern. Animal models play a crucial role in understanding the disease pathology and development of effective treatment strategies. Chemically induced CRC represents a cornerstone in animal model development; however, due to the presence of different animal species with different genetic backgrounds, it becomes mandatory to study the susceptibility of different mice species to CRC induction by different chemical entities such as 1,2-dimethylhydrazine (DMH). This study aimed to investigate the induction receptivity of two commonly used mice species, C57BL/6 and BALB/c, to DMH-induced CRC.
Both mice species were exposed to weekly intraperitoneal injections of DMH at a dose of 20 mg/kg body weight for 15 consecutive weeks. The response to DMH was evaluated by monitoring body weight gain, daily food intake, and gastrointestinal symptoms. At the end of exposure, histopathology of distal colon dissected from both species was analyzed.
Results revealed that C57BL/6 had a higher response to DMH compared to BALB/c. A significant decrease in body weight gain concomitant with severe diarrhea was observed in C57BL/6 receiving DMH compared to their controls, without any difference in food intake. Histopathology of distal colon revealed aberrant crypt foci and loss of goblet cells in DMH-exposed C57BL/6 mice. On the other hand, BALB/c mice displayed a normal and intact colon, with a normal weight gain pattern, and without any gastrointestinal symptoms.
In conclusion, C57BL/6 has a higher susceptibility toward chemical induction to CRC; therefore, it can be used to study CRC pathogenesis, prevention, and treatment.
结直肠癌（CRC）是一个重大的公共卫生问题。动物模型在理解疾病病理和制定有效治疗策略方面起着至关重要的作用。化学诱导的CRC是动物模型开发的基石；然而，由于存在具有不同遗传背景的不同动物物种，因此有必要研究不同小鼠物种对不同化学物质（如1,2 - 二甲基肼（DMH））诱导CRC的易感性。本研究旨在调查两种常用小鼠品系C57BL/6和BALB/c对DMH诱导的CRC的诱导反应性。
两种小鼠品系均连续15周每周腹腔注射剂量为20 mg/kg体重的DMH。通过监测体重增加、每日食物摄入量和胃肠道症状来评估对DMH的反应。暴露结束时，分析从两种小鼠品系解剖的远端结肠的组织病理学。
结果显示，与BALB/c相比，C57BL/6对DMH的反应更高。与对照组相比，接受DMH的C57BL/6小鼠体重增加显著下降，并伴有严重腹泻，食物摄入量无差异。远端结肠的组织病理学显示，暴露于DMH的C57BL/6小鼠出现异常隐窝灶和杯状细胞丢失。另一方面，BALB/c小鼠的结肠正常且完整，体重增加模式正常，无任何胃肠道症状。
总之，C57BL/6对化学诱导的CRC具有更高的易感性；因此，它可用于研究CRC的发病机制、预防和治疗。
