Ju Tian, Li Jixuan, Weston Andrew, Satta Giovanni, Bolognini Sara, Di Luca Mariagrazia, Gaisford Simon, Williams Gareth R
UCL School of Pharmacy, University College London, 29-39 Brunswick Square, London, WC1N 1AX, UK.
Centre for Clinical Microbiology, Royal Free Campus, University College London, Rowland Hill Street, London, NW3 2PF, UK.
Macromol Rapid Commun. 2025 Jul;46(13):e2400744. doi: 10.1002/marc.202400744. Epub 2025 Jan 13.
Antimicrobial resistance poses a growing threat to public health globally. Multidrug resistant Pseudomonas (P.) aeruginosa is detected in many infected wounds and is very challenging to treat with antibiotics. An alternative to antibiotics is to use bacteriophages, highly specific viruses able to kill even resistant bacteria. This work incorporates anti-P. aeruginosa Neko phages into monoaxial and coaxial electrospun fibers to explore their potential for treating infected wounds. Phages are blended with polyvinyl alcohol (PVA) solution and either processed directly into fibers or used as the core in coaxial electrospinning with polyvinylpyrrolidone (PVP) and PVP/ethyl cellulose (EC) shell solutions. Coaxial fibers stored at -20 °C show promising stability results, with negligible phage titer loss after 6 months of storage. Phage release can be controlled by varying the shell composition. Coaxial fibers with PVP as the shell (PVA/Su PVP + phage) demonstrate immediate release while fibers with PVP/EC as the shell (PVA/Su PVP/EC + phage) display extended-release. The antibacterial efficacy of phage lysate and phage-loaded fibers is studied by isothermal calorimetry and found to be unaffected by electrospinning. Thus, it appears that phage-loaded electrospun fibers merit further investigation as potential wound dressing materials.
抗菌耐药性对全球公共卫生构成了日益严重的威胁。在许多感染伤口中都检测到了多重耐药的铜绿假单胞菌，用抗生素治疗极具挑战性。抗生素的一种替代方法是使用噬菌体，噬菌体是一种高度特异性的病毒，甚至能够杀死耐药细菌。这项工作将抗铜绿假单胞菌的Neko噬菌体纳入单轴和同轴电纺纤维中，以探索其治疗感染伤口的潜力。噬菌体与聚乙烯醇（PVA）溶液混合，要么直接加工成纤维，要么在与聚乙烯吡咯烷酮（PVP）和PVP/乙基纤维素（EC）壳溶液的同轴电纺中用作核心。储存在-20°C的同轴纤维显示出良好的稳定性结果，储存6个月后噬菌体滴度损失可忽略不计。噬菌体的释放可以通过改变壳的组成来控制。以PVP为壳的同轴纤维（PVA/Su PVP+噬菌体）表现出即时释放，而以PVP/EC为壳的纤维（PVA/Su PVP/EC+噬菌体）则显示出缓释。通过等温量热法研究了噬菌体裂解液和载有噬菌体的纤维的抗菌效果，发现其不受电纺的影响。因此，载有噬菌体的电纺纤维作为潜在的伤口敷料材料似乎值得进一步研究。
