Liao Ning, Wang Juan, Liu Guanwen, Li Yinghui, Xu Fengqin, Xu Keyi, Shi Dingyu, Shao Dongyan, Jiang Chunmei, Shi Junling
Key Laboratory for Space Bioscience and Biotechnology, School of Life Sciences, Northwestern Polytechnical University, Xi'an, 710072, Shaanxi, People's Republic of China.
Food Engineering Technology Research Center/Key Laboratory of Henan Province, College of Food Science and Engineering, Henan University of Technology, Zhengzhou, 450001, Henan, People's Republic of China.
Food Funct. 2025 Feb 3;16(3):908-927. doi: 10.1039/d4fo04523h.
Inflammatory bowel disease (IBD) is a chronic inflammation with a high incidence rate. Many probiotics, including (), have shown promise in IBD treatment. The therapeutic effects of most probiotics are greatly decided by the available live cells in the disease lesion, which is compromised as they pass through the gastric juice and intestinal tract, resulting in a loss of activity. To improve probiotic delivery efficiency in the intestinal tract, broken spore shells (bGLS) were explored as a carrier to enhance the intestinal tract delivery of SHA113, a probiotic that has been verified to have capability to treat IBD. It was found the bGLS treated with iturin A and hydrochloric acid (IH-bGLS) had much higher affinity to probiotic cells than the untreated ones. This is possibly due to the enhancement of hydrophobic and positive charge of bGLS. Furthermore, IH-bGLS demonstrated an 81% loading efficiency for SHA113 and 2.2% for . More importantly, loading in IH-bGLS greatly enhanced the delivery of SHA113 cells to the colon and prolonged their retention time from 48 to over 120 h ( < 0.01). The mechanisms might be related to the enhancement of probiotic cell adhesion to the gastrointestinal mucosa, increase of mucus secretion and the upregulated expression of tight junction proteins, occludin and ZO-1, in the colon. The results of the animal experiment showed that the therapeutic effects of SHA113 on IBD were greatly enhanced when they were loaded with IH-bGLS. The novelty of this research is in the development of probiotic carriers from bGLS, which has significance in the improvement of intestinal delivery efficiency and the therapeutic effects of probiotics on IBD. This system may have attractive application in the enhancement of probiotic delivery efficiency in the intestinal tract, which is important to ensure and enhance the beneficial effects of probiotics.
炎症性肠病（IBD）是一种发病率较高的慢性炎症。许多益生菌，包括（），在IBD治疗中已显示出前景。大多数益生菌的治疗效果很大程度上取决于疾病病灶中可用的活细胞，而这些细胞在通过胃液和肠道时会受到损害，导致活性丧失。为了提高益生菌在肠道中的递送效率，人们探索了破碎的孢子壳（bGLS）作为载体，以增强已被证实具有治疗IBD能力的益生菌SHA113的肠道递送。研究发现，用伊枯草菌素A和盐酸处理的bGLS（IH-bGLS）对益生菌细胞的亲和力比未处理的bGLS高得多。这可能是由于bGLS的疏水性和正电荷增强所致。此外，IH-bGLS对SHA113的负载效率为81%，对（此处原文缺失相关内容）为2.2%。更重要的是，负载在IH-bGLS中的SHA113细胞向结肠的递送大大增强，其保留时间从48小时延长至超过120小时（P<0.01）。其机制可能与益生菌细胞对胃肠道黏膜的粘附增强、黏液分泌增加以及结肠中紧密连接蛋白occludin和ZO-1的表达上调有关。动物实验结果表明，当SHA113负载IH-bGLS时，其对IBD的治疗效果大大增强。这项研究的新颖之处在于从bGLS开发益生菌载体，这对于提高肠道递送效率和益生菌对IBD的治疗效果具有重要意义。该系统在提高肠道中益生菌递送效率方面可能具有诱人的应用前景，这对于确保和增强益生菌的有益效果非常重要。
