Kuang Xinwen, Liang Zhengyun, Xia Yijun, Shan Mengjie, Hao Yan, Liu Hao, Wang Zhi, He Qianjun, Xia Chao, Feng Cheng, Chang Guojing, Wang Youbin
Department of Plastic Surgery, Peking Union Medical College Hospital, Chinese Academy of Medical Science and Peking Union Medical College, Beijing, China.
Department of Dermatology, Shenzhen Center for Chronic Disease Control, Shenzhen Institute of Dermatology, Shenzhen, China.
J Cell Mol Med. 2025 Jan;29(1):e70292. doi: 10.1111/jcmm.70292.
Impaired wound healing affects the life quality of patients and causes a substantial financial burden. Hydrogen-rich medium is reported to have antioxidant and anti-inflammatory effects. However, the role of hydrogen-rich saline (HRS) in cutaneous wound healing remains largely unexplored, especially by metabolomics. Thus, untargeted metabolomics profiling was analysed to study the effects and mechanism of HRS combined with vacuum sealing drainage (VSD) in a rabbit full-thickness wound model. Our results indicated that the combination treatment of HRS and VSD could accelerate wound healing. In vitro experiments further confirmed its effects on HaCaT keratinocytes. We found that 45 metabolites were significantly changed between the VSD + HRS group and the VSD + saline-treated group. Pathway enrichment analysis indicated that biotin metabolism was the potential target pathway. The biochemical interpretation analysis demonstrated that combining HRS and VSD might enhance mitochondrial function, ATP synthesis, and GSH homeostasis by altering biotin metabolism. The detection of representative indicators of oxidative stress supported the critical metabolic pathway analysis as well. In summary, VSD combined with HRS might provide a new strategy to enhance wound healing.
伤口愈合受损会影响患者的生活质量，并造成巨大的经济负担。据报道，富氢培养基具有抗氧化和抗炎作用。然而，富氢盐水（HRS）在皮肤伤口愈合中的作用在很大程度上仍未得到探索，尤其是通过代谢组学方法。因此，我们通过非靶向代谢组学分析，研究了HRS联合封闭负压引流（VSD）在兔全层伤口模型中的作用及机制。我们的结果表明，HRS与VSD联合治疗可加速伤口愈合。体外实验进一步证实了其对HaCaT角质形成细胞的作用。我们发现，VSD+HRS组与VSD+盐水处理组之间有45种代谢物发生了显著变化。通路富集分析表明，生物素代谢是潜在的靶标通路。生化解释分析表明，HRS与VSD联合应用可能通过改变生物素代谢来增强线粒体功能、ATP合成和谷胱甘肽稳态。氧化应激代表性指标的检测也支持了关键代谢通路分析。总之，VSD联合HRS可能为促进伤口愈合提供一种新策略。
