Gao Hanxu, Xing Danjie, Wu Menglong, Hu Yilin, He Jiancheng, Chen Shun, Zhang Guangze, Yao Fangzhou, Ma Peng, Xue Wanjiang
Department of Gastrointestinal Surgery, Affiliated Hospital of Nantong University, Medical School of Nantong University, Nantong 226001, China.
Research Center of Clinical Medicine, Affiliated Hospital of Nantong University, Nantong 226001, China.
ACS Biomater Sci Eng. 2025 Feb 10;11(2):1171-1183. doi: 10.1021/acsbiomaterials.4c02043. Epub 2025 Jan 13.
Perianal fistulas (PAFs) are a severe complication of Crohn's disease that significantly impact patient prognosis and quality of life. While stem-cell-based strategies have been widely applied for PAF treatment, their efficacy remains limited. Our study introduces an injectable, temperature-controlled decellularized adipose tissue-alginate hydrogel loaded with dental pulp mesenchymal stem cells (DPMSCs) for in vivo fistula treatment. The experimental group demonstrated higher healing rates compared to surgical and DPMSCs groups, as evidenced by magnetic resonance imaging, multiplex immunohistochemical, and ELISA analyses. KEGG enrichment of differential genes suggested cellular senescence involvement in cell therapy efficacy, further confirmed by β-galactosidase staining and senescence markers (p21 and p53). Collectively, our research provides a novel therapy for PAFs and illuminates underlying mechanisms.
肛周瘘管（PAFs）是克罗恩病的一种严重并发症，会显著影响患者的预后和生活质量。虽然基于干细胞的治疗策略已广泛应用于PAF的治疗，但其疗效仍然有限。我们的研究引入了一种可注射的、温度可控的脱细胞脂肪组织-藻酸盐水凝胶，其负载牙髓间充质干细胞（DPMSCs）用于体内瘘管治疗。磁共振成像、多重免疫组化和ELISA分析表明，与手术组和DPMSCs组相比，实验组的愈合率更高。差异基因的KEGG富集分析表明细胞衰老与细胞治疗疗效有关，β-半乳糖苷酶染色和衰老标志物（p21和p53）进一步证实了这一点。总的来说，我们的研究为PAF提供了一种新的治疗方法，并阐明了其潜在机制。
