Memon Fareed Uddin, Zhu Yanqing, Cui Ying, Feng Xingbao, Ahmad Sheraz, Zeng Peng, Nabi Farhan, Hao Dengjian, Huang Zhijun, Tettamanti Gianluca, Tian Ling
Guangdong Provincial Key Lab of Agro-animal Genomics and Molecular Breeding, College of Animal Science, South China Agricultural University, Guangzhou 510642, China; Guangxi Key Laboratory of Sericulture Ecology and Applied Intelligent Technology/ Guangxi Collaborative Innovation Center of Modern Sericulture and Silk, Hechi University, Hechi 546300, China.
College of Natural Resources and Environment, South China Agricultural University, Guangzhou 510642, China.
Waste Manag. 2025 Feb 15;194:158-168. doi: 10.1016/j.wasman.2025.01.011. Epub 2025 Jan 12.
Sericulture waste poses significant challenges to industrial and environmental safety. Black soldier fly larvae (BSFL) offer a promising solution for organic waste management by converting it into insect protein. This study aimed to develop a microbial fermented method for utilizing sericulture waste to feed BSFL and explore the underlying mechanisms. Our results showed that all fermented sericulture waste groups had positive effects on body weight, survival rate, substrate consumption rate, and substrate conversion rate. Metagenomic analysis revealed a notable increase in the abundances of commensal genera, including Sedimentibacter, Clostridium, Enterococcus, Bacteroides, and Bacillus, in the gut of BSFL fed on sericulture waste fermented with the most effective combination of microbial strains (B. subtilis, B. licheniformis, and E. faecalis). In contrast, BSFL reared on unfermented sericulture waste exhibited higher abundances of potentially pathogenic and harmful genera, including Providencia, Klebsiella, Escherichia, Brucella, and Enterobacter. Clusters of orthologous genes (COG) analysis indicated that altered microbial communities in the fermented group mainly participated in metabolic pathways, defense mechanism, and signal transduction mechanism. Transcriptome analysis further revealed that the upregulated genes were functionally associated with key metabolic pathways and immune mechanisms in the fermented group. These findings underscore the pivotal role of selected microbial fermentation in utilizing sericulture waste as BSFL feed, providing a sustainable solution for organic waste management.
蚕桑废弃物对工业和环境安全构成重大挑战。黑水虻幼虫（BSFL）通过将其转化为昆虫蛋白，为有机废弃物管理提供了一个有前景的解决方案。本研究旨在开发一种利用蚕桑废弃物饲养BSFL的微生物发酵方法，并探索其潜在机制。我们的结果表明，所有发酵蚕桑废弃物组对体重、存活率、底物消耗率和底物转化率均有积极影响。宏基因组分析显示，在以最有效微生物菌株组合（枯草芽孢杆菌、地衣芽孢杆菌和粪肠球菌）发酵的蚕桑废弃物喂养的BSFL肠道中，共生菌属的丰度显著增加，包括沉积杆菌属、梭菌属、肠球菌属、拟杆菌属和芽孢杆菌属。相比之下，以未发酵蚕桑废弃物饲养的BSFL表现出较高丰度的潜在致病和有害菌属，包括普罗威登斯菌属、克雷伯菌属、大肠杆菌属、布鲁氏菌属和肠杆菌属。直系同源基因簇（COG）分析表明，发酵组中微生物群落的改变主要参与代谢途径、防御机制和信号转导机制。转录组分析进一步揭示，发酵组中上调的基因在功能上与关键代谢途径和免疫机制相关。这些发现强调了所选微生物发酵在利用蚕桑废弃物作为BSFL饲料方面的关键作用，为有机废弃物管理提供了一种可持续的解决方案。
