Rossi Tatiana Spatola, Francescato Luca, Gupte Ameya Pankaj, Favaro Lorenzo, Treu Laura, Campanaro Stefano
Department of Biology, University of Padova, 35131 Padova, Italy.
Waste to Bioproducts Lab, Department of Agronomy Food Natural Resources Animals and Environment, University of Padova - Agripolis, 35020 Legnaro, PD, Italy.
Bioresour Technol. 2025 Mar;419:132060. doi: 10.1016/j.biortech.2025.132060. Epub 2025 Jan 11.
The fermentation process in alcoholic beverage production converts sugars into ethanol and CO, releasing significant amounts of greenhouse gases. Here, Cupriavidus necator DSM 545 was grown autotrophically using gas derived from alcoholic fermentation, using a fed-batch bottle system. Nutrient starvation was applied to induce intracellular accumulation of poly(3-hydroxybutyrate) (PHB), a bioplastic polymer, for bioconversion of CO-rich waste gas into PHB. Grape marc, another by-product of wine production, was evaluated as a low-cost carbon source for the heterotrophic growth of C. necator, which was subsequently used as an inoculum for autotrophic cultures. The effect of agitation, CO headspace composition, and nitrogen concentration was tested, obtaining a maximum PHB concentration of 0.69 g/L, with an average CO uptake rate of 1.14 ± 0.41 mmol CO Lh and 65 % efficiency of CO consumption. These findings lay the groundwork for developing carbon mitigation strategies in alcoholic fermentation processes coupled with sustainable biopolymer production.
酒精饮料生产中的发酵过程将糖类转化为乙醇和二氧化碳，释放出大量温室气体。在此，利用分批补料瓶系统，以酒精发酵产生的气体为原料，自养培养食酸铜绿假单胞菌DSM 545。通过施加营养饥饿诱导细胞内积累聚（3-羟基丁酸酯）（PHB），这是一种生物塑料聚合物，用于将富含二氧化碳的废气生物转化为PHB。葡萄皮渣是葡萄酒生产的另一种副产品，被评估为食酸铜绿假单胞菌异养生长的低成本碳源，随后用作自养培养的接种物。测试了搅拌、二氧化碳顶空组成和氮浓度的影响，获得了0.69 g/L的最大PHB浓度，平均二氧化碳吸收速率为1.14±0.41 mmol CO/(L·h)，二氧化碳消耗效率为65%。这些发现为在酒精发酵过程中制定碳减排策略以及可持续生物聚合物生产奠定了基础。
