Fisher S J, Leitch M S, Kantor M S, Basbaum C B, Kramer R H
J Cell Biochem. 1985;27(1):31-41. doi: 10.1002/jcb.240270105.
First-trimester human placental villi were cultured on 3H-leucine-labeled extracellular matrices isolated from the PF HR9 and PYS-2 cell lines. Both cell lines produced an extracellular matrix that contained basement membrane-specific macromolecules, including type IV collagen, laminin and proteoglycan. Both matrices promoted outgrowth of cells from the villi which, according to morphological criteria, were identified as cytotrophoblastic cells. As the cells migrated from the attachment site, they caused a marked focal dissolution of the matrix which was accompanied by a concomitant release of 3H-labeled material into the media. Approximately half of this material chromatographed near the inclusion volume of Sephadex G-50, indicating that the labeled matrix components had been degraded. This phenomenon was dependent on the age of the placenta. Second-trimester placental villi also adhered to the matrix, but no areas of dissolution were formed and no significant amounts of radioactivity were released into the medium. These results suggest that culture of first-trimester human placental villi on extracellular matrices may be useful for the study of some of the early embryonic events leading to human implantation, during which the trophoblastic cells erode the uterine epithelium.
将孕早期人胎盘绒毛培养于从PF HR9和PYS - 2细胞系分离得到的用³H - 亮氨酸标记的细胞外基质上。这两种细胞系都产生了一种含有基底膜特异性大分子的细胞外基质，包括IV型胶原、层粘连蛋白和蛋白聚糖。两种基质都促进了绒毛细胞的生长，根据形态学标准，这些细胞被鉴定为细胞滋养层细胞。当细胞从附着部位迁移时，它们导致基质出现明显的局部溶解，同时伴随着³H标记物质释放到培养基中。大约一半的这种物质在Sephadex G - 50的包含体积附近层析，表明标记的基质成分已被降解。这种现象取决于胎盘的年龄。孕中期胎盘绒毛也能附着于基质，但未形成溶解区域，也没有大量放射性物质释放到培养基中。这些结果表明，在细胞外基质上培养孕早期人胎盘绒毛可能有助于研究导致人类着床的一些早期胚胎事件，在此期间滋养层细胞侵蚀子宫上皮。
