通过高效尺寸排阻色谱法快速分析淀粉、直链淀粉和支链淀粉。

Rapid analysis of starch, amylose and amylopectin by high-performance size-exclusion chromatography.

作者信息

Kobayashi S, Schwartz S J, Lineback D R

出版信息

J Chromatogr. 1985 Feb 1;319(2):205-14. doi: 10.1016/s0021-9673(01)90555-2.

Abstract

Starch components, amylose and amylopectin, were analyzed by high-performance size-exclusion chromatography. These two-components were separated using a two-column system (E-Linear and E-1000) and dimethyl sulphoxide as the mobile phase. The void volume (V0 = 2.22 ml) was measured using tobacco mosaic virus. Column calibration was accomplished with dextrans of known average molecular weight (Mw range = 10,100-2,000,000). The elution volume of amylopectin (Ve = 2.5 ml) indicated that this starch component was fractionated on the column system despite its very large molecular size. Standard curves were prepared from various mixtures of purified corn and wheat amylose and amylopectin. From the linear relationships obtained, the percentages of both components in corn and wheat starches were determined. The method developed proved useful to monitor the purity of amylose and amylopectin preparations, and to estimate rapidly the amylose:amylopectin ratio of starch samples.

摘要

采用高效尺寸排阻色谱法分析淀粉成分直链淀粉和支链淀粉。使用双柱系统（E-Linear和E-1000）和二甲基亚砜作为流动相来分离这两种成分。使用烟草花叶病毒测定空隙体积（V0 = 2.22 ml）。用已知平均分子量（Mw范围 = 10,100 - 2,000,000）的葡聚糖进行柱校准。支链淀粉的洗脱体积（Ve = 2.5 ml）表明，尽管该淀粉成分分子尺寸非常大，但在柱系统上仍被分级分离。由纯化的玉米和小麦直链淀粉与支链淀粉的各种混合物制备标准曲线。根据得到的线性关系，测定玉米和小麦淀粉中两种成分的百分比。所开发的方法被证明可用于监测直链淀粉和支链淀粉制剂的纯度，并快速估计淀粉样品的直链淀粉：支链淀粉比例。