Yang Yang, Hong Qing, Zhang Xuehong, Liu Zhenmin
State Key Laboratory of Dairy Biotechnology, Shanghai Engineering Research Center of Dairy Biotechnology, Shanghai, China.
Dairy Research Institute, Bright Dairy & Food Co., Ltd, Shanghai, China.
Food Funct. 2025 Feb 3;16(3):943-965. doi: 10.1039/d4fo05273k.
Rheumatoid arthritis (RA) is a systemic, chronic autoimmune disease. Many studies have shown that microorganisms may be an important pathological factor leading to the onset of RA. Some infectious or non-infectious pathogenic microorganisms and their metabolites may be the initiating factors of the early onset of RA. The aim of this study was to alleviate RA by regulating the imbalance of the gut microbiota in the early stage of RA using mixed bacterial strains. The mixed strains of (BD18, NCFM, BD1802, BD3545, BD5032) significantly reduced the clinical score and swelling thickness of the paws of collagen-induced arthritis (CIA) rats. The expression of TNF-α and MMP-13 proteins in the joints of CIA rats was also inhibited. The levels of specific antibodies (anti-CII IgG, anti-CII IgG1, anti-CII IgG2a, and anti-CII IgG2b) and inflammatory factor IL-6 in the serum of CIA rats were also significantly reduced. The relative abundance of , _UCG-014, , _, , , and ___1 in the gut microbiota of CIA rats receiving oral administration of the mixed strains of was significantly reduced, bringing their microbiota closer to that of healthy rats. Moreover, the levels of intestinal metabolites of short chain fatty acids in CIA rats, acetic acid and butyric acid, were significantly increased. The mixed strains of could recover the relative abundance of , _UCG-014_unclassified, , , and , affecting purine metabolism, transcription factors and alanine metabolism, and reducing the levels of specific antibodies and inflammatory factors in the blood, finally slowing the development of experimental arthritis induced by CII, which exerts the protective effect of probiotics. Mixed strains are more effective than a single strain, and the effect is not the result of a single strain, but of a combination of strains.
类风湿关节炎（RA）是一种全身性慢性自身免疫性疾病。许多研究表明，微生物可能是导致RA发病的重要病理因素。一些感染性或非感染性致病微生物及其代谢产物可能是RA早期发病的起始因素。本研究的目的是通过使用混合菌株调节RA早期肠道微生物群的失衡来缓解RA。（BD18、NCFM、BD1802、BD3545、BD5032）混合菌株显著降低了胶原诱导性关节炎（CIA）大鼠爪子的临床评分和肿胀厚度。CIA大鼠关节中TNF-α和MMP-13蛋白的表达也受到抑制。CIA大鼠血清中特异性抗体（抗CII IgG、抗CII IgG1、抗CII IgG2a和抗CII IgG2b）和炎性因子IL-6的水平也显著降低。口服该混合菌株的CIA大鼠肠道微生物群中_UCG-014、、、、和___1的相对丰度显著降低，使其微生物群更接近健康大鼠。此外，CIA大鼠肠道短链脂肪酸代谢产物乙酸和丁酸的水平显著升高。该混合菌株可恢复、UCG-014_unclassified、、、_和_的相对丰度，影响嘌呤代谢、转录因子和丙氨酸代谢，并降低血液中特异性抗体和炎性因子的水平，最终减缓由CII诱导的实验性关节炎的发展，发挥益生菌的保护作用。混合菌株比单一菌株更有效，其效果不是单一菌株的结果，而是菌株组合的结果。
