Li Xin, Qin Yanting, Yue Fangfang, Lü Xin
College of Food Science and Engineering, Northwest A&F University, Yangling, 712100 Shaanxi, China.
College of Life Sciences, Northwestern Polytechnical University, Xi'an, 710129 Shaanxi, China.
J Agric Food Chem. 2025 Jan 29;73(4):2381-2392. doi: 10.1021/acs.jafc.4c06638. Epub 2025 Jan 14.
Norvaline is a nonproteinogenic amino acid and an important food ingredient supplement for healthy food. In this study, dl-norvaline administration reduced body weight by more than 40% and improved glucose metabolism and energy metabolism in obese mice induced by a high-fat diet (HFD). Combination analysis of microbiome and metabolomics showed that dl-norvaline supplementation regulated gut bacteria structure, such as increasing beneficial bacteria (, , , , , , , and ) and decreasing harmful bacteria (, , , , , and ) and modulated the metabolites involved in arachidonic acid metabolism, thus further promoting short-chain fatty acid production and improving gut barrier, thereby inflammatory responses and oxidative stress were ameliorated. In addition, the pseudogerm-free mouse model verified that dl-norvaline ameliorated obesity-associated disorders in HFD-fed obese mice by targeting gut microbiota. These results clarified that dl-norvaline may be promising for developing and innovating potential functional food products.
正缬氨酸是一种非蛋白质氨基酸，是健康食品中重要的食品成分补充剂。在本研究中，给予dl-正缬氨酸可使高脂饮食（HFD）诱导的肥胖小鼠体重减轻40%以上，并改善其葡萄糖代谢和能量代谢。微生物组和代谢组学的联合分析表明，补充dl-正缬氨酸可调节肠道细菌结构，如增加有益菌（、、、、、、和）和减少有害菌（、、、、和），并调节花生四烯酸代谢相关的代谢物，从而进一步促进短链脂肪酸的产生并改善肠道屏障，进而减轻炎症反应和氧化应激。此外，无菌小鼠模型证实，dl-正缬氨酸通过靶向肠道微生物群改善了HFD喂养的肥胖小鼠的肥胖相关疾病。这些结果表明，dl-正缬氨酸在开发和创新潜在功能性食品方面可能具有前景。
