Chen Runsen, Zhang Yuxi, Shen Yang, Wu Kede, Mo Xuming, Yang Zhaocong
Department of Cardiothoracic Surgery, Children's Hospital of Nanjing Medical University, Nanjing, 210008, China.
Animal Core Facility, Nanjing Medical University, Nanjing, 210008, China.
J Neuroimmune Pharmacol. 2025 Jan 14;20(1):7. doi: 10.1007/s11481-024-10168-0.
Parkinson's disease (PD) is a complex progressive neurodegenerative disorder and the pathogenesis and treatment methods are unknown. This aim is to investigate the effects of long non coding RNA NEAT1 (LncRNA NEAT1) on 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP)-induced Parkinson's disease (PD). Immunoprecipitation and western blot were used to search for the effects of LncRNA NEAT1 on PD. Tyrosine hydroxylase (TH) and brain derived neurotrophic factor (BDNF) were evaluated in substantia nigra (SN) region of the brain by immunohistochemical staining. Compared with the control group, the relative expression level of LncRNA NEAT1 in the MPTP group was significantly increased. LncRNA NEAT1 is negatively correlated with miR-376b-3p. LncRNA NEAT1 significantly increased oxidative stress, neuroinflammation along with enhanced neurotrophic potential via NLR family Pyrin domain protein 3 (NLRP3) pathway. In conclusion, these results indicated that LncRNA NEAT1 participated in the pathophysiological of PD and its mechanism via the miR-376b-3p/NLRP3 signaling pathway.
帕金森病（PD）是一种复杂的进行性神经退行性疾病，其发病机制和治疗方法尚不清楚。本研究旨在探讨长链非编码RNA NEAT1（LncRNA NEAT1）对1-甲基-4-苯基-1,2,3,6-四氢吡啶（MPTP）诱导的帕金森病（PD）的影响。采用免疫沉淀和蛋白质印迹法研究LncRNA NEAT1对PD的影响。通过免疫组织化学染色评估脑黑质（SN）区域的酪氨酸羟化酶（TH）和脑源性神经营养因子（BDNF）。与对照组相比，MPTP组中LncRNA NEAT1的相对表达水平显著升高。LncRNA NEAT1与miR-376b-3p呈负相关。LncRNA NEAT1通过NLR家族含pyrin结构域蛋白3（NLRP3）途径显著增加氧化应激、神经炎症，并增强神经营养潜能。总之，这些结果表明LncRNA NEAT1通过miR-376b-3p/NLRP3信号通路参与PD的病理生理过程及其机制。
