Xie Sizhe, Chen Yichun, Guo Jing, Liu Yulong, Liu Yanqin, Fan Jiyong, Wang Hairui, Wu Jianfeng, Xie Jianwei
Laboratory of Toxicant Analysis, Academy of Military Medical Sciences, Beijing 100850, China.
Laboratory of Toxicant Analysis, Academy of Military Medical Sciences, Beijing 100850, China; Key Laboratory of Preparation and Applications of Environmental Friendly Materials, Ministry of Education, Jilin Normal University, Changchun 130103, China.
J Hazard Mater. 2025 Apr 5;487:137150. doi: 10.1016/j.jhazmat.2025.137150. Epub 2025 Jan 7.
Organophosphorus nerve agents (OPNAs) are highly lethal chemical warfare agents (CWAs), which poses a serious threat to human health and safety. The accurate and rapid identification of OPNAs is crucial for medical diagnosis and effective treatment. However, distinguishing between various OPNAs and their analogues using on-site point-of-care testing (POCT) remains challenging. Herein, we present a novel Raman-enhanced strategy that employs a chemical capture probe through a structural differential amplification derivative probe coupled with handheld Raman spectrometry. In this method, 2-(dimethylamino methyl)-3-hydroxypyridine (2-DMAMPD) was designed and used to capture target OPNAs in the plasmonic hotspot for the first time. The formation of strong Au-N bonds between nanoparticles and pyridine significantly enhances the cross-section and specific Raman intensity of OPNAs, facilitating effective amplification and differentiation of subtle structural variations among different OPNAs. In practical application, the probe solution can be directly sprayed on the surfaces contaminated by agents, allowing the entire detection process to be completed within five minutes, with a detection limit of 2 ng/mL (equivalent to an absolute content of 50 pg). It is worth noting that during the process of detection, highly toxic OPNAs can be quickly transformed into low-toxic or non-toxic derivatives, which is of great significance for green detection and protection of the operator.
有机磷神经毒剂（OPNAs）是极具杀伤力的化学战剂（CWAs），对人类健康与安全构成严重威胁。准确快速地识别OPNAs对于医学诊断和有效治疗至关重要。然而，利用现场即时检测（POCT）区分各种OPNAs及其类似物仍然具有挑战性。在此，我们提出一种新型的拉曼增强策略，该策略通过结构差异放大衍生探针结合手持式拉曼光谱法使用化学捕获探针。在该方法中，首次设计并使用2-(二甲基氨基甲基)-3-羟基吡啶（2-DMAMPD）在等离子体热点中捕获目标OPNAs。纳米颗粒与吡啶之间形成的强Au-N键显著增强了OPNAs的截面和特定拉曼强度，有助于有效放大和区分不同OPNAs之间细微的结构差异。在实际应用中，可将探针溶液直接喷洒在被毒剂污染的表面，整个检测过程可在五分钟内完成，检测限为2 ng/mL（相当于50 pg的绝对含量）。值得注意的是，在检测过程中，高毒性的OPNAs可迅速转化为低毒或无毒衍生物，这对于绿色检测和保护操作人员具有重要意义。
