Wu Zhen, Wang Jinlong, Cheng Xing, Tang Yue, Xia Lian, Niu Xiaojuan, Wu Yuangen
Key Laboratory of Plant Resource Conservation and Germplasm Innovation in Mountainous Region (Ministry of Education), College of Life Sciences/Institute of Agro-bioengineering, Guizhou University, Guiyang 550025, China.
Guizhou Xijiu Co. Ltd., Xishui 564622, China.
Food Chem. 2025 Apr 15;471:142837. doi: 10.1016/j.foodchem.2025.142837. Epub 2025 Jan 9.
Nanozyme-based sensors for detecting ascorbic acid (AA) generally depend on the reducibility of the analyte. However, these sensors are susceptible to interference from reducing substances in food. Herein, a novel fluorescent sensor for AA detection was developed based on inhibiting the phosphatase-like activity of a Zr-based metal-organic framework (Zr-CAU-28). Hydroxyl-rich AA molecules adsorb on the surface of Zr-CAU-28 through hydrogen bonding with [ZrO(OH)] cluster, leading to a decrease in the relative content of terminal hydroxyl groups within the catalytic sites. The constructed sensor exhibits a wide detection range (0.08-11 μg·mL) and low detection limit of 0.025 μg·mL. Potential interfering studies demonstrated the good selectivity of the sensor. Moreover, the fluorescent sensor can effectively detect AA in juices and vitamin C tablets, with the recovery rate ranging from 96.25 % to 108.50 %. This work represents the first application of phosphatase mimics for AA detection, offering a new strategy for food analysis.
基于纳米酶的抗坏血酸（AA）检测传感器通常依赖于分析物的还原性。然而，这些传感器容易受到食品中还原性物质的干扰。在此，基于抑制锆基金属有机框架（Zr-CAU-28）的磷酸酶样活性，开发了一种用于检测AA的新型荧光传感器。富含羟基的AA分子通过与[ZrO(OH)]簇形成氢键吸附在Zr-CAU-28表面，导致催化位点内末端羟基的相对含量降低。构建的传感器具有宽检测范围（0.08-11μg·mL）和0.025μg·mL的低检测限。潜在干扰研究证明了该传感器具有良好的选择性。此外，该荧光传感器能够有效检测果汁和维生素C片中的AA，回收率在96.25%至108.50%之间。这项工作代表了磷酸酶模拟物在AA检测中的首次应用，为食品分析提供了一种新策略。
