Li Fang, Wang Chen, Zhao Zhengzheng, Yang Chongrui, Gao Bingqi, Yu Ziniu, Zhang Jibin, Cai Minmin, Yu Chan
State Key Laboratory of Biocatalysis and Enzyme Engineering, School of Life Sciences, Hubei University, Wuhan 430062 China; State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070 China; Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China.
State Key Laboratory of Agricultural Microbiology, National Engineering Research Center of Microbial Pesticides, College of Life Science and Technology, Huazhong Agricultural University, Wuhan 430070 China; Hubei Hongshan Laboratory, Wuhan, Hubei 430070, China.
Bioresour Technol. 2025 Mar;419:132067. doi: 10.1016/j.biortech.2025.132067. Epub 2025 Jan 12.
To effectively address the contamination caused by antibiotic misuse, this study was conducted to enhance the removal of amoxicillin (AMX) and penicillin sodium (PEN) by incorporating black soldier fly larvae (BSFL). The results showed that BSFL increased the degradation rates of AMX and PEN to 71.00 % and 80.89 %, respectively, and shortened their half-lives to 238 h and 160 h. Proteobacteria (26.2 %-82.0 %), Firmicutes (13.3 %-54.0 %), Acinobacteriota (2.1 %-23.4 %), and Bacteroidota (1.3 %-10.1 %) were the intestinal dominant microorganisms during transformation. Five bacteria with β-lactam antibiotic resistance in the BSFL gut were isolated, among which Morganella morganii demonstrated strong antibiotic tolerance and high removal rates of AMX and PEN in both in vitro and in vivo experiments, ranging from 58.99 % to 95.87 %. BSFL intestinal bacteria disrupted the quaternary pharmacophore of AMX and PEN, breaking them down into at least seven and five metabolites, respectively.
为有效解决抗生素滥用造成的污染问题，本研究通过引入黑水虻幼虫（BSFL）来提高阿莫西林（AMX）和青霉素钠（PEN）的去除效果。结果表明，黑水虻幼虫分别将AMX和PEN的降解率提高到71.00%和80.89%，并将它们的半衰期缩短至238小时和160小时。变形菌门（26.2%-82.0%）、厚壁菌门（13.3%-54.0%）、放线菌门（2.1%-23.4%）和拟杆菌门（1.3%-10.1%）是转化过程中的肠道优势微生物。从黑水虻幼虫肠道中分离出5株具有β-内酰胺抗生素抗性的细菌，其中摩根氏摩根菌在体外和体内实验中均表现出较强的抗生素耐受性以及较高的AMX和PEN去除率，范围为58.99%至95.87%。黑水虻幼虫肠道细菌破坏了AMX和PEN的四级药效团，分别将它们分解为至少7种和5种代谢产物。
