Chen Xufeng, Tang Chao, Cai Dixin, Yin Qing, Xie Qian, Xu Pengfang, Huang Lina
Department of Orthopedics, Wuhan Hankou Hospital, Wuhan, 430014, China.
Department of Orthopedics, Shanghai Eighth People's Hospital, Shanghai 200235, China.
J Formos Med Assoc. 2025 Jan 13. doi: 10.1016/j.jfma.2025.01.003.
Osteoporosis fracture is a common and most serious complication of osteoporosis.
This study sought to assess the level, the diagnostic potential, and the effect of circulating miR-4534 in osteoporotic fractures.
GSE74209 and GSE93883 were analyzed using GEO2R online tool for differentially expressed microRNAs in osteoporotic fractures. Postmenopausal women were recruited and serum samples were determined by RT-qPCR for level of miR-4534. ROC curves were plotted to evaluate the diagnostic value of miR-4534 in osteoporotic fractures. The effects of miR-4534 on hFOB 1.19 osteogenic marker levels, proliferation, and migration were measured by RT-qPCR, CCK-8 assay and Transwell assay, respectively. The target gene for miR-4534 was predicted and rescue experiments were conducted.
miR-4534 was identified as an upregulated miR in osteoporotic fracture. Up-regulated miR-4534 had the potential to distinguish osteoporotic fracture from health, and from common osteoporosis. The up-regulated miR-4534 in hFOB 1.19 cocultured with human umbilical vein endothelial cells could inhibit cell osteogenic marker levels, proliferation and migration. CFTR was a target gene of miR-4534 and rescued the suppression of miR-4534 on hFOB 1.19 activity.
MiR-4534 is a new potential diagnostic biomarker for osteoporotic fractures. MiR-4534 can regulate osteoblast differentiation, proliferation, and migration by targeting CFTR.
骨质疏松性骨折是骨质疏松症常见且最严重的并发症。
本研究旨在评估循环miR - 4534在骨质疏松性骨折中的水平、诊断潜力及作用。
使用GEO2R在线工具分析GSE74209和GSE93883，以确定骨质疏松性骨折中差异表达的微小RNA。招募绝经后女性，通过RT - qPCR测定血清样本中miR - 4534的水平。绘制ROC曲线以评估miR - 4534在骨质疏松性骨折中的诊断价值。分别通过RT - qPCR、CCK - 8测定法和Transwell测定法检测miR - 4534对hFOB 1.19成骨标志物水平、增殖和迁移的影响。预测miR - 4534的靶基因并进行挽救实验。
miR - 4534被鉴定为骨质疏松性骨折中上调的miR。上调的miR - 4534有潜力区分骨质疏松性骨折与健康状态以及普通骨质疏松症。与人类脐静脉内皮细胞共培养的hFOB 1.19中上调的miR - 4534可抑制细胞成骨标志物水平、增殖和迁移。CFTR是miR - 4534的靶基因，并挽救了miR - 4534对hFOB 1.19活性的抑制作用。
MiR - 4534是骨质疏松性骨折的一种新的潜在诊断生物标志物。MiR - 4534可通过靶向CFTR调节成骨细胞的分化、增殖和迁移。
