Diagnostic value and fracture healing-preventing effect of upregulated microRNA-4534 in patients with osteoporotic fractures.

BACKGROUND

Osteoporosis fracture is a common and most serious complication of osteoporosis.

HYPOTHESIS

This study sought to assess the level, the diagnostic potential, and the effect of circulating miR-4534 in osteoporotic fractures.

METHODS

GSE74209 and GSE93883 were analyzed using GEO2R online tool for differentially expressed microRNAs in osteoporotic fractures. Postmenopausal women were recruited and serum samples were determined by RT-qPCR for level of miR-4534. ROC curves were plotted to evaluate the diagnostic value of miR-4534 in osteoporotic fractures. The effects of miR-4534 on hFOB 1.19 osteogenic marker levels, proliferation, and migration were measured by RT-qPCR, CCK-8 assay and Transwell assay, respectively. The target gene for miR-4534 was predicted and rescue experiments were conducted.

RESULTS

miR-4534 was identified as an upregulated miR in osteoporotic fracture. Up-regulated miR-4534 had the potential to distinguish osteoporotic fracture from health, and from common osteoporosis. The up-regulated miR-4534 in hFOB 1.19 cocultured with human umbilical vein endothelial cells could inhibit cell osteogenic marker levels, proliferation and migration. CFTR was a target gene of miR-4534 and rescued the suppression of miR-4534 on hFOB 1.19 activity.

CONCLUSIONS

MiR-4534 is a new potential diagnostic biomarker for osteoporotic fractures. MiR-4534 can regulate osteoblast differentiation, proliferation, and migration by targeting CFTR.