大鼠单克隆抗体。III. 一种促进体内杂交瘤细胞生长的简单方法。

Rat monoclonal antibodies. III. A simple method for facilitation of hybridoma cell growth in vivo.

作者信息

Hirsch F, Vendeville B, De Clercq L, Bazin H, Druet P

出版信息

J Immunol Methods. 1985 Apr 8;78(1):103-8. doi: 10.1016/0022-1759(85)90333-3.

Abstract

Hybridoma cells that did not grow when injected subcutaneously or intraperitoneally in histocompatible or Rnu/Rnu rats were injected intravenously into histocompatible recipients. Eight of the 9 cell lines injected in this way grew in several organs of the recipient 3-7 weeks later. Hybridoma cells proliferated mainly in the liver. When the liver homogenate of these animals was injected intraperitoneally into histocompatible recipients, hybridoma cells grew readily giving rise to ascites containing the expected monoclonal antibody for 6 of the 9 cell lines.

摘要

将在组织相容性或Rnu/Rnu大鼠中皮下或腹腔注射时不生长的杂交瘤细胞静脉注射到组织相容性受体中。以这种方式注射的9个细胞系中有8个在3至7周后在受体的几个器官中生长。杂交瘤细胞主要在肝脏中增殖。当将这些动物的肝脏匀浆腹腔注射到组织相容性受体中时，杂交瘤细胞很容易生长，9个细胞系中有6个产生了含有预期单克隆抗体的腹水。