Duwadi Achyut, Sapkota Surya, Zou Cheng, Chen Li-Ling, Cadle-Davidson Lance, Hwang Chin-Feng
Plant Science Graduate Program, Missouri State University, Springfield, MO 65897, U.S.A.
USDA-ARS, Appalachian Fruit Research Station, Kearneysville, WV 25430, U.S.A.
Plant Dis. 2025 Jul;109(7):1470-1477. doi: 10.1094/PDIS-10-24-2133-RE. Epub 2025 Jul 10.
Powdery mildew, caused by the fungus , is one of the primary causes of grape yield loss across the globe. Although numerous resistance loci have been identified in various grapevine species, the genetic determinants of susceptibility to remain largely unexplored. Understanding the genetics of susceptibility for pathogenesis is equally important for developing durable resistance grapevines against this pathogen. To identify these factors in interspecific hybrid 'Chambourcin,' a controlled leaf disc assay was conducted for 2 years using an automated microscopy phenotyping system with 273 F genotypes from a cross of 'Chambourcin' and 'Cabernet Sauvignon.' Additionally, a high-resolution linkage map using the same number of 'Chambourcin'-derived hybrids was constructed with 355 simple sequence repeats and 1,394 RNaseH2-dependent amplicon sequencing-derived haplotype markers that clustered into 19 linkage groups. A quantitative trait locus analysis identified a susceptibility locus (here named ) on linkage group 7 explaining 8.90 to 12.57% of the total phenotypic variance. The markers associated with this susceptibility locus were used to identify 78 accessions in the U.S. Department of Agriculture Agricultural Research Service cold-hardy collection at Geneva, NY, that carry and can be used to selectively exclude susceptible progenies. Additionally, six accessions carry the alternative haplotype encoding recessive resistance and can be used for resistance breeding. The identification of powdery mildew susceptibility loci is crucial for identifying genes that could be targeted for gene knockout, gene editing, RNA interference, or selection among breeding genotypes to enhance sustainable protection against pathogens.
白粉病由真菌引起，是全球葡萄产量损失的主要原因之一。尽管在各种葡萄品种中已鉴定出许多抗性基因座，但对该真菌易感性的遗传决定因素仍 largely unexplored。了解发病机制易感性的遗传学对于培育对这种病原体具有持久抗性的葡萄同样重要。为了在种间杂交品种“尚博辛”中鉴定这些因素，使用自动显微镜表型分析系统对来自“尚博辛”和“赤霞珠”杂交的273个F基因型进行了为期2年的受控叶盘试验。此外，利用相同数量的源自“尚博辛”的杂交品种构建了一张高分辨率连锁图谱，该图谱包含355个简单序列重复标记和1394个基于核糖核酸酶H2依赖性扩增子测序的单倍型标记，这些标记聚集成19个连锁群。数量性状基因座分析在第7连锁群上鉴定出一个易感性基因座（此处命名为），解释了总表型变异的8.90%至12.57%。与该易感性基因座相关的标记用于在美国纽约州日内瓦的美国农业部农业研究服务处抗寒葡萄品种收集库中鉴定78个携带该基因座的品种，这些品种可用于选择性排除易感后代。此外，有六个品种携带编码隐性抗性的替代单倍型，可用于抗性育种。白粉病易感性基因座的鉴定对于识别可用于基因敲除、基因编辑、RNA干扰或在育种基因型中进行选择以增强对病原体的可持续保护的基因至关重要。
