Niu Jin-Wei, Zhang Guo-Chao, Ning Wu, Liu Hai-Bin, Yang Hua, Li Chao-Feng
Department of General Surgery, China-Japan Friendship Hospital, Beijing 100029, China.
World J Gastroenterol. 2025 Jan 14;31(2):97239. doi: 10.3748/wjg.v31.i2.97239.
The objective of the current study was to elucidate the clinical mechanism through which phospholipase D2 (PLD2) exerted a regulatory effect on neutrophil migration, thereby alleviating the progression of acute pancreatitis.
To elucidate the clinical mechanism through which PLD2 exerted a regulatory effect on neutrophil migration, thereby alleviating the progression of acute pancreatitis.
The study involved 90 patients diagnosed with acute pancreatitis, admitted to our hospital between March 2020 and November 2022. A retrospective analysis was conducted, categorizing patients based on Ranson score severity into mild ( = 25), moderate ( = 30), and severe ( = 35) groups. Relevant data was collected for each group. Western blot analysis assessed PLD2 protein expression in patient serum. Real-time reverse transcription polymerase chain reaction was used to evaluate the mRNA expression of chemokine receptors associated with neutrophil migration. Serum levels of inflammatory factors in patients were detected using enzyme-linked immunosorbent assay. Transwell migration tests were conducted to compare migration of neutrophils across groups and analyze the influence of PLD2 on neutrophil migration.
Overall data analysis did not find significant differences between patient groups ( > 0.05). The expression of PLD2 protein in the severe group was lower than that in the moderate and mild groups ( < 0.05). The expression level of PLD2 in the moderate group was also lower than that in the mild group ( < 0.05). The severity of acute pancreatitis is negatively correlated with PLD2 expression ( = -0.75, = 0.002). The mRNA levels of C-X-C chemokine receptor type 1, C-X-C chemokine receptor type 2, C-C chemokine receptor type 2, and C-C chemokine receptor type 5 in the severe group are significantly higher than those in the moderate and mild groups ( < 0.05), and the expression levels in the moderate group are also higher than those in the mild group ( < 0.05). The levels of C-reactive protein, tumor necrosis factor-α, interleukin-1β, and interleukin-6 in the severe group were higher than those in the moderate and mild groups ( < 0.05), and the levels in the moderate group were also higher than those in the mild group ( < 0.05). The number of migrating neutrophils in the severe group was higher than that in the moderate and mild groups ( < 0.05), and the moderate group was also higher than the mild group ( < 0.05). In addition, the number of migrating neutrophils in the mild group combined with PLD2 inhibitor was higher than that in the mild group ( < 0.05), and the number of migrating neutrophils in the moderate group combined with PLD2 inhibitor was higher than that in the moderate group ( < 0.05). The number of migrating neutrophils in the severe group + PLD2 inhibitor group was significantly higher than that in the severe group ( < 0.05), indicating that PLD2 inhibitors significantly stimulated neutrophil migration.
PLD2 exerted a crucial regulatory role in the pathological progression of acute pancreatitis. Its protein expression varied among patients based on the severity of the disease, and a negative correlation existed between PLD2 expression and disease severity. Additionally, PLD2 appeared to impede acute pancreatitis progression by limiting neutrophil migration.
本研究的目的是阐明磷脂酶D2（PLD2）对中性粒细胞迁移发挥调节作用从而减轻急性胰腺炎进展的临床机制。
阐明PLD2对中性粒细胞迁移发挥调节作用从而减轻急性胰腺炎进展的临床机制。
本研究纳入了2020年3月至2022年11月期间我院收治的90例急性胰腺炎患者。进行回顾性分析，根据兰森评分严重程度将患者分为轻度（=25）、中度（=30）和重度（=35）组。收集每组的相关数据。采用蛋白质免疫印迹分析评估患者血清中PLD2蛋白表达。使用实时逆转录聚合酶链反应评估与中性粒细胞迁移相关的趋化因子受体的mRNA表达。采用酶联免疫吸附测定法检测患者血清中炎症因子水平。进行Transwell迁移试验以比较各组中性粒细胞的迁移情况，并分析PLD2对中性粒细胞迁移的影响。
总体数据分析未发现患者组间存在显著差异（>0.05）。重度组PLD2蛋白表达低于中度组和轻度组（<0.05）。中度组PLD2表达水平也低于轻度组（<0.05）。急性胰腺炎的严重程度与PLD2表达呈负相关（=-0.75，=0.002）。重度组C-X-C趋化因子受体1型、C-X-C趋化因子受体2型、C-C趋化因子受体2型和C-C趋化因子受体5型的mRNA水平显著高于中度组和轻度组（<0.05），中度组的表达水平也高于轻度组（<0.05）。重度组C反应蛋白、肿瘤坏死因子-α、白细胞介素-1β和白细胞介素-6水平高于中度组和轻度组（<0.05），中度组水平也高于轻度组（<0.05）。重度组迁移的中性粒细胞数量高于中度组和轻度组（<0.05），中度组也高于轻度组（<0.05）。此外，轻度组联合PLD2抑制剂后迁移的中性粒细胞数量高于轻度组（<0.05），中度组联合PLD2抑制剂后迁移的中性粒细胞数量高于中度组（<0.05）。重度组+PLD2抑制剂组迁移的中性粒细胞数量显著高于重度组（<0.05），表明PLD2抑制剂显著刺激中性粒细胞迁移。
PLD2在急性胰腺炎的病理进展中发挥关键调节作用。其蛋白表达因疾病严重程度在患者中有所不同，且PLD2表达与疾病严重程度呈负相关。此外，PLD2似乎通过限制中性粒细胞迁移来阻碍急性胰腺炎的进展。
