Tsong Y Y, Chang C C, Nash H A
J Reprod Immunol. 1985 Feb;7(2):139-49. doi: 10.1016/0165-0378(85)90068-3.
The antibody response obtained after vaccinating rabbits with the beta-subunit of human chorionic gonadotropin (beta-hCG) linked to several protein and polysaccharide carriers was measured. In all but one preparation, carbodiimide was used to couple the beta-hCG to the carrier. Tetanus toxoid (TT) and cholera vaccine proved the most effective carriers among those examined. TT from different manufacturers proved to be greatly different in free amino group content and differed in ability to participate in the coupling reaction. Reasonably good replication of the coupling reaction was obtained with different production lots from the same manufacturer. Inferior antigenic response was obtained with the products of coupling beta-hCG to H. pertussis, influenza vaccine, polylysine, pneumococcus polysaccharide, or E. coli polysaccharide. The findings indicate TT and cholera vaccine to be especially effective in enhancing the antigenicity of a weakly antigenic peptide but point to significant differences in the TT from different manufacturers.
测定了用与几种蛋白质和多糖载体相连的人绒毛膜促性腺激素β亚基（β-hCG）对兔子进行免疫接种后获得的抗体反应。除一种制剂外，其余所有制剂均使用碳二亚胺将β-hCG与载体偶联。破伤风类毒素（TT）和霍乱疫苗被证明是所检测载体中最有效的。来自不同制造商的TT在游离氨基含量上有很大差异，并且在参与偶联反应的能力上也有所不同。使用同一制造商不同生产批次的产品可获得相当良好的偶联反应重复性。将β-hCG与百日咳杆菌、流感疫苗、聚赖氨酸、肺炎球菌多糖或大肠杆菌多糖偶联的产物获得的抗原反应较差。这些发现表明TT和霍乱疫苗在增强弱抗原性肽的抗原性方面特别有效，但也指出不同制造商生产的TT存在显著差异。
