Hachiman Masaki, Kuroda Hiroki
Graduate School of Media and Governance, Keio University, Kanagawa, Japan.
Faculty of Environment and Information Studies, Keio University, Kanagawa, Japan.
MicroPubl Biol. 2024 Dec 30;2024. doi: 10.17912/micropub.biology.001448. eCollection 2024.
In , axial elongation beyond the tailbud stage requires gamma-aminobutyric acid (GABA). However, the role of GABA synthesized during early development in this process remains unclear. In this study, by treating embryos with allylglycine (AG), an inhibitor of GABA synthesis, we observed a significant reduction in axial elongation. This inhibition was rescued by exogenous GABA, demonstrating that GABA synthesis via glutamate decarboxylase (GAD) is essential for axial elongation after the tailbud stage. Our findings suggest that GABA-dependent elongation functions independently of mechanisms like convergent extension, which are crucial during early development.
在[具体情况未提及]中，尾芽期之后的轴伸长需要γ-氨基丁酸（GABA）。然而，早期发育过程中合成的GABA在此过程中的作用仍不清楚。在本研究中，通过用GABA合成抑制剂烯丙基甘氨酸（AG）处理胚胎，我们观察到轴伸长显著减少。外源性GABA挽救了这种抑制作用，表明通过谷氨酸脱羧酶（GAD）合成GABA对于尾芽期之后的轴伸长至关重要。我们的研究结果表明，GABA依赖的伸长独立于早期发育过程中至关重要的诸如汇聚延伸等机制发挥作用。
