Fernández-Carro Estibaliz, Ciriza Jesús
Tissue Microenvironment (TME) Lab, Aragón Institute of Engineering Research (I3A), University of Zaragoza, Zaragoza, Spain.
Department of Anatomy and Histology, Faculty of Medicine, University of Zaragoza, Zaragoza, Spain.
Methods Mol Biol. 2025 Jan 18. doi: 10.1007/7651_2024_579.
In vitro skin aging models represent a valuable tool for the study of age-related pathologies and potential treatments. However, the currently available models do not adequately represent the complex microenvironment of the dermis since they generally focus on cutaneous cellular senescence, rather than the full range of factors that contribute to the aging process, such as structural and compositional alteration of the dermal extracellular matrix. The following protocol describes the extraction and characterization of human adult extracellular matrix scaffolds for use in in vitro aging models.
体外皮肤衰老模型是研究与年龄相关的病理状况和潜在治疗方法的宝贵工具。然而，目前可用的模型并不能充分代表真皮的复杂微环境，因为它们通常侧重于皮肤细胞衰老，而不是导致衰老过程的所有因素，如真皮细胞外基质的结构和成分改变。以下方案描述了用于体外衰老模型的成人细胞外基质支架的提取和表征。
