Clinical diagnostic value of PIMREG on liver cancer cell phenotype and tumorigenic ability in nude mice.

OBJECTIVES

In vitro experiments were manipulated to investigate the effect of the (PICALM-interacting mitotic regulator gene) expression level on the malignant phenotype of liver cancer cells and their tumorigenesis ability in nude mice, and bioinformatics were used to analyze the clinical diagnostic and prognostic value in liver cancer.

METHODS

After liver cancer-related data were obtained from the TCGA database and GTEx database, the differences in expression in liver cancer and normal liver tissue were compared using bioinformatics, and their correlation with the clinical pathological characteristics of liver cancer and the prognosis value were analyzed. A knockdown and overexpression model of was constructed using Huh7 cells. The effect of the expression level on the malignant phenotype of Huh7 cells was tested through CCK-8 and Transwell experiments. At the same time, animal knockdown and overexpression models were constructed to study the effect of the expression level on the tumorigenesis ability in nude mice.

RESULTS

Bioinformatics analysis showed that mRNA was significantly overexpressed in liver cancer tissue (<0.001). There were differences in T-staging (<0.001), pathological staging (=0.002), vascular infiltration (<0.001), histological grading (<0.001), and AFP levels (<0.001) between the high- and low-expression groups. A high expression of is associated with a poor prognosis, manifested as a significant decrease in the overall survival, disease-specific survival, and progression-free survival rates of patients ( values of 0.006, 0.014, and 0.002, respectively). In the overexpression model, the proliferation rate and invasion ability of Huh7 cells were significantly increased, and the tumorigenesis ability of nude mice was significantly enhanced. In the knockdown model, the opposite results were observed.

CONCLUSIONS

The gene is highly expressed in hepatocellular carcinoma, and increasing its expression level can significantly promote the malignant phenotype of liver cancer cells and their tumorigenesis ability in nude mice. Knocking down its expression level has the opposite effect. The expression level of is related to the pathological stages of liver cancer patients, and its elevated expression is a risk factor for poor prognosis. may become a new target for the clinical diagnosis, treatment, and prognosis evaluation of liver cancer.