Huang Ziteng, Jia Keran, Tan Yadan, Yu Yang, Xiao Wudian, Zhou Xiangyu, Yi Jingyan, Zhang Chunxiang
Department of Anesthesiology, The Affiliated Hospital, Southwest Medical University, Luzhou, 646000, Sichuan, China.
Department of Medical Cell Biology and Genetics, School of Basic Medical Sciences, Southwest Medical University, Luzhou, 646000, Sichuan, China.
Cardiovasc Diabetol. 2025 Jan 18;24(1):25. doi: 10.1186/s12933-025-02598-8.
Globally, cardiovascular diseases remain among the leading causes of mortality, highlighting the urgent need for innovative research models. Consequently, the development of accurate models that simulate cardiac function holds significant scientific and clinical value for both disease research and therapeutic interventions. Cardiac organoids, which are three-dimensional structures derived from the induced differentiation of stem cells, are particularly promising. These organoids not only replicate the autonomous beating and essential electrophysiological properties of the heart but are also widely employed in studies related to cardiac diseases, drug efficacy testing, and regenerative medicine. This review comprehensively surveys the various fabrication techniques used to create cardiac organoids and their diverse applications in modeling a range of cardiac diseases. We emphasize the role of advanced technologies in enhancing the maturation and functionality of cardiac cells, ensuring that these models closely resemble native cardiac tissue. Furthermore, we discuss monitoring techniques and evaluation parameters critical for assessing the performance of cardiac organoids, considering the complex interactions within multi-organ systems. This approach is vital for enhancing precision and efficiency in drug development, allowing for more effective therapeutic strategies. Ultimately, this review aims to provide a thorough and innovative perspective on both fundamental research and clinical treatment of cardiovascular diseases, offering insights that could pave the way for future advancements in understanding and addressing these prevalent health challenges.
在全球范围内，心血管疾病仍然是主要的死亡原因之一，这凸显了对创新研究模型的迫切需求。因此，开发能够模拟心脏功能的精确模型对于疾病研究和治疗干预具有重要的科学和临床价值。心脏类器官是由干细胞诱导分化产生的三维结构，尤其具有前景。这些类器官不仅能复制心脏的自主跳动和基本电生理特性，还广泛应用于心脏病相关研究、药物疗效测试和再生医学。本综述全面调查了用于创建心脏类器官的各种制造技术及其在模拟一系列心脏病方面的多样应用。我们强调先进技术在增强心脏细胞成熟度和功能方面的作用，确保这些模型与天然心脏组织极为相似。此外，考虑到多器官系统内的复杂相互作用，我们讨论了对评估心脏类器官性能至关重要的监测技术和评估参数。这种方法对于提高药物开发的精度和效率至关重要，能够实现更有效的治疗策略。最终，本综述旨在为心血管疾病的基础研究和临床治疗提供全面且创新的视角，提供的见解可为未来在理解和应对这些普遍存在的健康挑战方面的进展铺平道路。
