Lu Zonglin, Zhao Haiyang, Wang Hui, Wang Xin, Sun Zixue
The Second Clinical Medical College, Henan University of Traditional Chinese Medicine, No. 7, Jiudu Road, Xigong District, Zhengzhou, Henan, 450008, China.
Infertility Department, Beijing University of Traditional Chinese Medicine Dongzhimen Hospital Luoyang Hospital, Luoyang, Henan, 471000, China.
Basic Clin Androl. 2025 Jan 20;35(1):2. doi: 10.1186/s12610-024-00247-w.
To investigate the therapeutic potential of Acrorus tatarinowii in oligoasthenozoospermia and its related mechanism through modulation of the BCL2/Bax/Caspase3 signaling pathway.
Initially, using the TCMSP and Disgenet databases, active ingredients of Acrorus tatarinowii were identified and their target genes associated with sperm-related diseases were elucidated.Subsequently, an oligoasthenozoospermia mouse model was induced and treated with Acrorus tatarinowii. Serum hormone levels were assessed by ELISA, testicular histopathology by HE staining, and target gene expression by qPCR and Western blotting.
Acrorus tatarinowii treatment significantly upregulated BCL2 expression in the testes of oligoasthenozoospermic rat. This was accompanied by improved histopathological features in testicular tissues, reduced LH and FSH levels in serum.
Acrorus tatarinowii exerts therapeutic effects in oligoasthenozoospermia by regulating the BCL2/Bax/Caspase3 pathway, maybe by inhibiting apoptosis, and promoting germ cell proliferation. These findings highlight its potential as a natural remedy for male infertility associated with sperm function disorders.
通过调节BCL2/Bax/Caspase3信号通路，研究石菖蒲对少弱精子症的治疗潜力及其相关机制。
首先，利用中药系统药理学数据库与分析平台（TCMSP）和DisGeNET数据库，鉴定石菖蒲的活性成分，并阐明其与精子相关疾病相关的靶基因。随后，建立少弱精子症小鼠模型并用石菖蒲进行治疗。通过酶联免疫吸附测定（ELISA）评估血清激素水平，通过苏木精-伊红（HE）染色评估睾丸组织病理学，通过定量聚合酶链反应（qPCR）和蛋白质免疫印迹法检测靶基因表达。
石菖蒲治疗显著上调了少弱精子症大鼠睾丸中BCL2的表达。同时，睾丸组织的组织病理学特征得到改善，血清中促黄体生成素（LH）和促卵泡生成素（FSH）水平降低。
石菖蒲通过调节BCL2/Bax/Caspase3通路对少弱精子症发挥治疗作用，可能是通过抑制细胞凋亡和促进生殖细胞增殖。这些发现凸显了其作为治疗与精子功能障碍相关男性不育症天然药物的潜力。
