Kim Han-Ul, Kim Young Kwan
Department of Biochemistry, College of Natural Sciences, Kangwon National University, Chuncheon, 24341, Republic of Korea.
Kangwon Center for Systems Imaging, Kangwondaehak-gil, Chuncheon-si, Gangwon-do, 24341, Republic of Korea.
Appl Microsc. 2025 Jan 20;55(1):1. doi: 10.1186/s42649-024-00106-y.
The development of bispecific antibodies (BsAbs) represents a significant advancement in therapeutic antibody design, enabling the simultaneous targeting of two different antigens. This dual-targeting capability enhances therapeutic efficacy, particularly in complex diseases like cancer, where tumor heterogeneity presents a significant challenge for traditional treatments. By bridging two distinct pathways, BsAbs can improve specificity and minimize off-target effects, making them invaluable in therapeutic contexts. Integrating advanced imaging techniques, particularly Correlative Light and Electron Microscopy (CLEM), offers a unique opportunity to visualize the dynamic interactions of BsAbs within cellular environments. CLEM combines the strengths of optical and electron microscopy, allowing researchers to observe real-time antibody-antigen interactions at nanoscale resolution. This synergy not only deepens our understanding of BsAbs' mechanisms of action but also provides critical insights into their spatial distribution, binding kinetics, and functional dynamics in live cells. In this review, the integration of BsAbs and CLEM paves the way for targeted therapeutic strategies, fostering the development of more effective treatments that can adapt to the complexities of disease pathology.
双特异性抗体（BsAbs）的发展代表了治疗性抗体设计的重大进步，能够同时靶向两种不同的抗原。这种双靶向能力增强了治疗效果，特别是在癌症等复杂疾病中，肿瘤异质性对传统治疗构成了重大挑战。通过连接两条不同的途径，双特异性抗体可以提高特异性并将脱靶效应降至最低，使其在治疗环境中具有极高价值。整合先进的成像技术，特别是相关光电子显微镜（CLEM），为可视化双特异性抗体在细胞环境中的动态相互作用提供了独特的机会。CLEM结合了光学显微镜和电子显微镜的优势，使研究人员能够在纳米级分辨率下观察实时抗体 - 抗原相互作用。这种协同作用不仅加深了我们对双特异性抗体作用机制的理解，还为其在活细胞中的空间分布、结合动力学和功能动态提供了关键见解。在这篇综述中，双特异性抗体与CLEM的整合为靶向治疗策略铺平了道路，促进了能够适应疾病病理复杂性的更有效治疗方法的发展。
