Regulation of volume reabsorption by thyroid hormones in the proximal tubule of rat: minor role of luminal sodium permeability.

In order to investigate whether changes in luminal membrane sodium permeability can explain the increase in isotonic fluid reabsorption (Jv) found in proximal tubules of thyroidectomized rats (TX) treated with tri-iodothyronine (T3), experiments were carried out on TX rats and TX rats treated for 3 days (TX + T3) with physiological doses (10 micrograms/kg body wt) of T3. Two sets of experiments were performed: 1) in vivo, using the micropuncture technique for the measurements of Jv; 2) in vitro, using isolated brush border membrane vesicles for the direct measurement of Na+ permeability. In micropuncture studies a 65% increase in Jv of TX rats was observed after treatment with T3. Luminal perfusion of proximal tubules of TX rats with Amphotericin B (10 micrograms/ml), to increase luminal sodium permeability, enhanced Jv only by 15%. Brush border membrane vesicles isolated from TX and TX + T3 rats showed the same sodium permeability in uptake or efflux experiments. These results were confirmed by the fact that sodium gradient dependent histidine transport into brush border membrane vesicles did not change after T3 treatment. Finally, measuring the amiloride sensitive sodium uptake, it was also found that Na+-H+ exchange was also only slightly affected by T3. These micropuncture and vesicle data indicate that the large effect of T3 on the trans-cellular sodium transport and volume reabsorption in the proximal tubule, cannot be explained by an action of T3 on the sodium entry step across the brush border membrane.