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伴有脊柱转移的乙状结肠癌类器官的建立与鉴定

Establishment and characterization of a sigmoid colon cancer organoid with spinal metastasis.

作者信息

Chen Jingyao, Cheng Shumin, Gu Liang, Huang Zhangsen, Zhang Changhua, Sun Chunhui, Chen Songyao

机构信息

Digestive Diseases Center, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, China.

Guangdong Provincial Key Laboratory of Digestive Cancer Research, The Seventh Affiliated Hospital of Sun Yat-sen University, Shenzhen, China.

出版信息

Front Cell Dev Biol. 2025 Jan 3;12:1510264. doi: 10.3389/fcell.2024.1510264. eCollection 2024.

DOI:10.3389/fcell.2024.1510264
PMID:39830210
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC11739105/
Abstract

BACKGROUND

Sigmoid colon cancer with spinal metastases is rare in distant metastasis. In addition, the prognosis of colon cancer patients with spinal metastases is extremely poor. In order to find effective therapeutic agents, we need to know the biological characteristics of such patients from related models.

METHODS

We collected sigmoid colon cancer tissue from a young female subject who was diagnosed with sigmoid colon cancer with multiple spinal metastases. We successfully established a sigmoid colon cancer organoid using this tissue and investigated drug screening in the patient. HE staining, immunohistochemistry, and DNA sequencing were utilized to compare the biological characteristics between the original tumor and the organoid. Furthermore, we investigated the drug screening of the sigmoid colon cancer organoid .

RESULTS

A colon cancer organoid from sigmoid colon cancer with spinal metastases was successfully established. The organoid culture maintained the morphological features, histological features, and genomic landscape of the corresponding sigmoid colon cancer cells. Moreover, we performed drug screening tests to evaluate the effects of chemotherapeutic drugs and targeted drugs.

CONCLUSION

The sigmoid colon cancer organoid with spinal metastases was a favorable preclinical model to explore the clinicopathologic characteristics of colon cancer patients with spinal metastases.

摘要

背景

乙状结肠癌伴脊柱转移在远处转移中较为罕见。此外，乙状结肠癌伴脊柱转移患者的预后极差。为了找到有效的治疗药物，我们需要从相关模型中了解此类患者的生物学特征。

方法

我们从一名被诊断为乙状结肠癌伴多发脊柱转移的年轻女性受试者身上收集了乙状结肠癌组织。我们利用该组织成功建立了乙状结肠癌类器官，并对该患者进行了药物筛选。采用苏木精-伊红染色、免疫组织化学和DNA测序来比较原发肿瘤与类器官之间的生物学特征。此外，我们还对乙状结肠癌类器官进行了药物筛选。

结果

成功建立了乙状结肠癌伴脊柱转移的结肠癌类器官。类器官培养保留了相应乙状结肠癌细胞的形态特征、组织学特征和基因组格局。此外，我们进行了药物筛选试验，以评估化疗药物和靶向药物的效果。

结论

乙状结肠癌伴脊柱转移的类器官是探索乙状结肠癌伴脊柱转移患者临床病理特征的良好临床前模型。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/316dad1ea004/fcell-12-1510264-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/1c62a51028f1/fcell-12-1510264-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/f7cbbc0aef33/fcell-12-1510264-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/9278afeb909b/fcell-12-1510264-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/bd0e353ace6a/fcell-12-1510264-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/e4475a260b4d/fcell-12-1510264-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/5ecbc2d6a34a/fcell-12-1510264-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/7e443fd0ab6c/fcell-12-1510264-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/519bb6532625/fcell-12-1510264-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/316dad1ea004/fcell-12-1510264-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/1c62a51028f1/fcell-12-1510264-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/f7cbbc0aef33/fcell-12-1510264-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/9278afeb909b/fcell-12-1510264-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/bd0e353ace6a/fcell-12-1510264-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/e4475a260b4d/fcell-12-1510264-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/5ecbc2d6a34a/fcell-12-1510264-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/7e443fd0ab6c/fcell-12-1510264-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/519bb6532625/fcell-12-1510264-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/403a/11739105/316dad1ea004/fcell-12-1510264-g009.jpg

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