Lockette W E, Webb R C
Proc Soc Exp Biol Med. 1985 Apr;178(4):536-45. doi: 10.3181/00379727-178-42039.
This study characterizes vascular responsiveness to sodium arachidonate (C 20:4) in four models of hypertension [deoxycorticosterone acetate (DOCA) hypertensive rats, two kidney-one clip (2K-1C) renal hypertensive rats, spontaneously hypertensive rats (SHR), and psychosocial hypertensive mice]. Isolated arterial strips (aorta, mesenteric artery, tail artery) were equilibrated under optimal resting tension in physiological salt solution for measurement of isometric force generation. Dose-response curves to arachidonate (10(-10) to 10(-4) g/ml) in arteries from DOCA and 2K-1C hypertensive rats were shifted to the left compared to those in arteries from control rats. In arteries from SHR and psychosocial hypertensive mice, the dose-response relationships were unchanged compared to normotensive values. Arteries from DOCA hypertensive and 2K-1C hypertensive rats developed greater maximal contractile responses to arachidonate than controls; maximal responses in arteries from SHR and psychosocial hypertensive mice were unchanged compared to normotensive values. Contractions to arachidonate were inhibited by indomethacin (0.5 and 5 micrograms/ml) and by aspirin (5 and 50 micrograms/ml). The fatty acid, oleate (C 18:1), had no effect on the contractile state of the arteries, whereas prostaglandin F2 alpha caused contraction. These results indicate altered responsiveness to exogenous arachidonate in arteries from DOCA and 2K-1C hypertensive rats, but not in arteries from SHR and psychosocial hypertensive mice.
本研究在四种高血压模型[醋酸去氧皮质酮（DOCA）高血压大鼠、二肾一夹（2K-1C）肾性高血压大鼠、自发性高血压大鼠（SHR）和心理社会应激性高血压小鼠]中，对血管对花生四烯酸钠（C20:4）的反应性进行了表征。将离体动脉条（主动脉、肠系膜动脉、尾动脉）在生理盐溶液中以最佳静息张力平衡，以测量等长力的产生。与对照大鼠动脉相比，DOCA和2K-1C高血压大鼠动脉对花生四烯酸（10(-10)至10(-4) g/ml）的剂量反应曲线向左移动。与正常血压值相比，SHR和心理社会应激性高血压小鼠动脉中的剂量反应关系未发生变化。DOCA高血压和2K-1C高血压大鼠的动脉对花生四烯酸产生的最大收缩反应比对照组更大；与正常血压值相比，SHR和心理社会应激性高血压小鼠动脉中的最大反应未发生变化。吲哚美辛（0.5和5微克/毫升）和阿司匹林（5和50微克/毫升）可抑制对花生四烯酸的收缩反应。脂肪酸油酸（C18:1）对动脉的收缩状态没有影响，而前列腺素F2α可引起收缩。这些结果表明，DOCA和2K-1C高血压大鼠的动脉对外源性花生四烯酸的反应性发生了改变，但SHR和心理社会应激性高血压小鼠的动脉则未改变。
