墨西哥乳腺癌患者血浆中循环游离核DNA和线粒体DNA的分析

Analysis of circulating cell-free nuclear and mitochondrial DNA in plasma of Mexican patients with breast cancer.

作者信息

Rodríguez-Torres Dalia E, Saucedo-Sariñana Anilu M, Palacios-Ramírez Alejandra, Godínez-Rodríguez Miriam Y, Salas-González Efraín, Alcaraz-Wong Aldo A, García-Ortiz José E, Rosales-Reynoso Mónica A

机构信息

División de Medicina Molecular, Centro de Investigación Biomédica de Occidente, Instituto Mexicano del Seguro Social, Guadalajara.

Dirección Académica Aparatos y Sistemas I, Facultad de Medicina. Decanato Ciencias de la Salud, Universidad Autónoma de Guadalajara, Zapopan.

出版信息

Gac Med Mex. 2024;160(4):404-412. doi: 10.24875/GMM.M24000917.

DOI:10.24875/GMM.M24000917

PMID:39832332

Abstract

BACKGROUND

The usefulness of circulating free DNA (cfDNA), nuclear DNA (nDNA) and mitochondrial DNA (mtDNA) as potential biomarkers in cancer remains controversial.

OBJECTIVE

To determine the concentration of cfDNA and plasma nDNA and mtDNA levels in breast cancer (BC) patients.

MATERIAL AND METHODS

This study included a total of 86 women (69 patients with BC and 17 women as a control group). The cfDNA was determined using fluorometry, and the levels of mtDNA and nDNA were quantified using real-time polymerase chain reaction (qPCR).

RESULTS

The concentration of cfDNA was higher in patients with BC compared to the control group (p = 0.001). A higher concentration of cfDNA was also associated with patients over 50 years old (p = 0.001). The level of nDNA was higher in patients with CM compared to the control group (p = 0.004). Levels of mtDNA did not show significant differences (p > 0.005). The concentration of cfDNA and nDNA showed significant discriminatory power (AUC = 0.968, CI = 0.93-1.00, p = 0.001; and AUC = 0.724, CI = 0.59-0.85, p = 0.004, respectively.

CONCLUSIONS

The concentration of cfDNA and the combination of cfDNA and nDNA are useful biomarkers for diagnosing patients with BC.

摘要

背景

循环游离DNA（cfDNA）、核DNA（nDNA）和线粒体DNA（mtDNA）作为癌症潜在生物标志物的实用性仍存在争议。

目的

测定乳腺癌（BC）患者的cfDNA浓度以及血浆nDNA和mtDNA水平。

材料与方法

本研究共纳入86名女性（69例BC患者和17名女性作为对照组）。使用荧光测定法测定cfDNA，使用实时聚合酶链反应（qPCR）对mtDNA和nDNA水平进行定量。

结果

与对照组相比，BC患者的cfDNA浓度更高（p = 0.001）。cfDNA浓度较高也与50岁以上患者相关（p = 0.001）。与对照组相比，CM患者的nDNA水平更高（p = 0.004）。mtDNA水平未显示出显著差异（p > 0.005）。cfDNA和nDNA浓度显示出显著的鉴别能力（AUC分别为0.968，CI = 0.93 - 1.00，p = 0.001；以及AUC = 0.724，CI = 0.59 - 0.85，p = 0.004）。