Wang Junyang, Ji Xu, Yi Renhe, Li Dengbin, Shen Xiaolin, Liu Zihe, Xia Yaying, Shi Shuobo
Beijing Advanced Innovation Center for Soft Matter Science and Engineering, College of Life Science and Technology, Beijing University of Chemical Technology, Beijing, China.
State Key Laboratory of Microbial Resources, Institute of Microbiology, Chinese Academy of Sciences, Beijing, China.
Biotechnol J. 2025 Jan;20(1):e202400712. doi: 10.1002/biot.202400712.
Terpenoids are widely distributed in nature and have various applications in healthcare products, pharmaceuticals, and fragrances. Despite the significant potential that terpenoids possess, traditional production methods, such as plant extraction and chemical synthesis, face challenges in meeting current market demand. With the advancement of synthetic biology and metabolic engineering, it becomes feasible to construct efficient microbial cell factories for large-scale production of terpenoids. This article primarily centers on the heterologous expression of terpenoids in Saccharomyces cerevisiae, detailing the expression of terpenoid biosynthesis pathways through the utilization of cellular microcompartments, strategies for the efficient expression of key P450 enzymes in the synthesis pathway, and the regulation and optimization of host metabolism to enhance flux to terpenoids synthesis. Additionally, we analyze current challenges and propose solutions to further refine yeast chassis for more effective terpenoids production.
萜类化合物在自然界中广泛分布，在保健品、药品和香料等领域有多种应用。尽管萜类化合物具有巨大的潜力，但传统的生产方法，如植物提取和化学合成，在满足当前市场需求方面面临挑战。随着合成生物学和代谢工程的发展，构建高效的微生物细胞工厂用于大规模生产萜类化合物变得可行。本文主要围绕萜类化合物在酿酒酵母中的异源表达展开，详细阐述了通过利用细胞微区室表达萜类生物合成途径、合成途径中关键P450酶高效表达的策略，以及宿主代谢的调控和优化以增强萜类化合物合成通量。此外，我们分析了当前面临的挑战并提出解决方案，以进一步优化酵母底盘，实现更有效的萜类化合物生产。
