Sun Meng, Yu Yiru, Zhang Weixing, Ding Yi, Li Ang, Li Ye
Key Laboratory of Shaanxi Province for Craniofacial Precision Medicine Research, College of Stomatology, Xi'an Jiaotong University, Xi'an, China.
Department of Periodontology, College of Stomatology, Xi'an Jiaotong University, Xi'an, Shaanxi, China.
Front Cell Dev Biol. 2024 Dec 20;12:1503481. doi: 10.3389/fcell.2024.1503481. eCollection 2024.
Tooth eruption as a crucial part in tooth development and regeneration is accompanied by ongoing osteogenesis and osteoclast activity. The dental follicle (DF) surrounding the developing tooth harbors dental follicle stem cells (DFSCs) which play a crucial role in maintaining bone remodeling. However, the mechanisms through which they regulate the balance between osteogenesis and osteoclast activity during tooth eruption remain poorly understood. Notably, extracellular vesicles (EVs) in bone homeostasis are considered essential. Our study revealed that the DFSCs could modulate tooth eruption by inhibiting osteoclast differentiation via EVs. Further investigation showed that EVs from DFSCs could inhibit osteoclast differentiation through the ANXA1-PPARγ-CEBPα pathway. Animal experiments indicated that EVs from DFSCs and the cargo ANXA1 affected tooth eruption. In summary, this study suggests the critical role of the dental follicle in tooth eruption through EVs, which may provide therapeutic targets for abnormal tooth eruption and effective approaches for the eruption of regenerated teeth.
牙齿萌出作为牙齿发育和再生的关键部分，伴随着持续的成骨作用和破骨细胞活性。发育中牙齿周围的牙囊（DF）含有牙囊干细胞（DFSCs），它们在维持骨重塑中起关键作用。然而，在牙齿萌出过程中，它们调节成骨作用和破骨细胞活性之间平衡的机制仍知之甚少。值得注意的是，细胞外囊泡（EVs）在骨稳态中被认为是必不可少的。我们的研究表明，DFSCs可以通过EVs抑制破骨细胞分化来调节牙齿萌出。进一步的研究表明，来自DFSCs的EVs可以通过ANXA1-PPARγ-CEBPα途径抑制破骨细胞分化。动物实验表明，来自DFSCs的EVs和货物ANXA1会影响牙齿萌出。总之，本研究表明牙囊通过EVs在牙齿萌出中起关键作用，这可能为异常牙齿萌出提供治疗靶点，并为再生牙齿的萌出提供有效方法。
