Islam Aminul, Sultan Md Zakir, Ali Md Ashraf, Mia Md Lemon, Salam Md Abdus
Department of Chemistry, University of Dhaka, Dhaka-1000, Bangladesh.
Center for Advanced Research in Sciences (CARS), University of Dhaka, Dhaka-1000, Bangladesh.
Heliyon. 2024 Dec 20;11(1):e41419. doi: 10.1016/j.heliyon.2024.e41419. eCollection 2025 Jan 15.
Thiamine (vitamin B1) and riboflavin (vitamin B2) are essential vitamins because of their important health impact. Twelve different green leafy vegetable-samples including , and indigenous to Shariatpur district in Bangladesh were collected, dried, stored and analyzed. The extraction procedures involved acid hydrolysis followed by enzymatic hydrolysis of the dried vegetable samples. A Shimadzu Prominence HPLC system operating in isocratic elution mode on a C18 column (4.6 mm × 250 mm, 5 μm) was utilized to achieve chromatographic separation of the vitamins while a fluorescence detector recorded the signals. The outcome showed that the HPLC method offered excellent sensitivity and selectivity for thiamine and riboflavin determination in leafy vegetables. The calibration curves exhibited good linearity (R > 0.99), and the recovery percentages were found to be excellent also, at 108.22 % and 100.22 % for thiamine and riboflavin, respectively. Comparative analyses revealed variations in thiamine and riboflavin contents in different leafy vegetables, highlighting the importance of including a diverse range of leafy vegetables in the diet to meet recommended daily intake levels of these vitamins.
硫胺素（维生素B1）和核黄素（维生素B2）因其对健康的重要影响而成为必需维生素。采集了包括孟加拉国沙里亚特布尔地区本土的12种不同绿叶蔬菜样本，进行干燥、储存和分析。提取过程包括对干燥蔬菜样本进行酸水解，然后进行酶水解。使用一台岛津Prominence高效液相色谱系统，在C18柱（4.6毫米×250毫米，5微米）上以等度洗脱模式运行，以实现维生素的色谱分离，同时用荧光检测器记录信号。结果表明，该高效液相色谱法在测定绿叶蔬菜中的硫胺素和核黄素时具有出色的灵敏度和选择性。校准曲线显示出良好的线性（R>0.99），硫胺素和核黄素的回收率也分别高达108.22%和100.22%，效果极佳。对比分析揭示了不同绿叶蔬菜中硫胺素和核黄素含量的差异，突出了在饮食中摄入多种绿叶蔬菜以满足这些维生素每日推荐摄入量的重要性。
