Li Yiran, Xu Hongxing, Zhao Wenwen, Zhang Li, Wu Shaohua
College of Textiles & Clothing, Qingdao University, Qingdao 266071, People's Republic of China.
Textile and Clothing College Experimental Teaching Center, Qingdao University, Qingdao 266071, People's Republic of China.
Biofabrication. 2025 Jan 30;17(2). doi: 10.1088/1758-5090/adacaf.
The design and development of advanced surgical sutures with appropriate structure and abundant bio-functions are urgently required for the chronic wound closure and treatment. In this study, an integrated technique routine combining modified electrospinning with hot stretching process was proposed and implemented to fabricate poly(L-lactic acid) (PLLA) nanofiber sutures, and the Salvia miltiorrhiza Bunge-Radix Puerariae herbal compound (SRHC) was encapsulated into PLLA nanofibers during the electrospinning process to enrich the biofunction of as-generated sutures. All the PLLA sutures loading without or with SRHC were found to exhibit bead-free and highly-aligned nanofiber structure. The addition of SRHC was found to have no significant influences on the fiber morphology, diameter, and the crystallinity of as-prepared PLLA sutures. Importantly, all the SRHC-contained PLLA nanofiber sutures possessed excellent tensile and knot strength, which were of significant importance for the surgical suture applications. Besides, the antioxidant and anti-inflammatory properties of these sutures obviously enhanced with the increasing of SRHC concentration. Furthermore, thecell tests illustrated that the high fiber orientation of the sutures was able to efficiently induce the human dermal fibroblasts (HDFs) to migrate in a rapid manner, and the sutures loaded with high content of SRHC could significantly promote the attachment and proliferation of HDFs in comparison. Thediabetic mouse model experiments revealed that all the as-developed PLLA sutures could effectively close the wound, but the PLLA sutures containing high content of SRHC could dramatically promote the wound healing with high quality by shortening the healing time, improving the collagen deposition, neovascularization, and the regeneration of hair follicles, especially compared with commercial polyester (PET) suture. This study offers a simple and easily-handling strategy to develop robust, biodegradable, bioactive, and nanostructured PLLA sutures, which shows huge potential for the treatment of hard-to-heal diabetic wounds.
慢性伤口的闭合和治疗迫切需要设计和开发具有适当结构和丰富生物功能的先进手术缝线。在本研究中，提出并实施了一种将改进的静电纺丝与热拉伸工艺相结合的集成技术流程，以制备聚（L-乳酸）（PLLA）纳米纤维缝线，并在静电纺丝过程中将丹参-葛根复方（SRHC）包封到PLLA纳米纤维中，以丰富所制备缝线的生物功能。结果发现，所有未添加或添加了SRHC的PLLA缝线均呈现无珠且高度排列的纳米纤维结构。结果发现，添加SRHC对所制备的PLLA缝线的纤维形态、直径和结晶度没有显著影响。重要的是，所有含SRHC的PLLA纳米纤维缝线均具有优异的拉伸强度和打结强度，这对于手术缝线应用具有重要意义。此外，随着SRHC浓度的增加，这些缝线的抗氧化和抗炎性能明显增强。此外，细胞测试表明，缝线的高纤维取向能够有效地诱导人皮肤成纤维细胞（HDFs）快速迁移，相比之下，负载高含量SRHC的缝线能够显著促进HDFs的附着和增殖。糖尿病小鼠模型实验表明，所有所开发的PLLA缝线都能有效闭合伤口，但含高含量SRHC的PLLA缝线能够通过缩短愈合时间、改善胶原蛋白沉积、新血管形成和毛囊再生，显著促进高质量的伤口愈合，尤其是与商业聚酯（PET）缝线相比。本研究提供了一种简单且易于操作的策略来开发坚固、可生物降解、具有生物活性的纳米结构PLLA缝线，其在治疗难愈合的糖尿病伤口方面显示出巨大潜力。
