Diaz Katherinne J, Jarquin Julian, Petrosyan Aleksandr, Takahashi Melissa K
Biology Department, California State University, Northridge, Northridge, California, United States.
MicroPubl Biol. 2025 Jan 2;2025. doi: 10.17912/micropub.biology.001334. eCollection 2025.
RNA toehold switches are powerful tools that can be used as biosensors to detect nearly any RNA sequence. In the presence of a specific RNA trigger, the toehold switch allows translation of a reporter protein. Toehold switches expressed in cell-free expression systems have been used as biosensors for several viruses and bacterial RNAs. Here we explore the use of the NanoLuc complementation reporter NanoBiT and found that it provides improved sensitivity over sfGFP. This reporter can be applied broadly across other RNA toehold switch applications.
RNA 触发开关是强大的工具，可用作生物传感器来检测几乎任何 RNA 序列。在特定 RNA 触发物存在的情况下，触发开关允许报告蛋白的翻译。在无细胞表达系统中表达的触发开关已被用作多种病毒和细菌 RNA 的生物传感器。在这里，我们探索了纳米荧光素酶互补报告基因 NanoBiT 的用途，发现它比超折叠绿色荧光蛋白（sfGFP）具有更高的灵敏度。该报告基因可广泛应用于其他 RNA 触发开关应用中。
