采用新型单克隆免疫放射分析法定量检测HBeAg/抗-HBe

HBeAg/anti-HBe determination with a new monoclonal immunoradiometric assay.

作者信息

Pizzocolo G, Giammatteo D, Barbieri U, Boniolo A, Callegaro L, Palla M, Albertini A

出版信息

Vox Sang. 1985;48(3):129-35. doi: 10.1111/j.1423-0410.1985.tb00159.x.

DOI:10.1111/j.1423-0410.1985.tb00159.x

Abstract

The performance of two assays for the HBeAg/anti-HBe system associated with hepatitis B virus infection, using monoclonal (EBK-Sorin) and polyclonal (HBe-Abbott) antibodies, has been compared. The results on a random population (1,000 samples) demonstrated for the monoclonal reagent a higher sensitivity, without any loss in specificity, and with the further advantage of the use of lower radioactivity levels. The proportion of positives and negatives obtained with the two kits was found to remain unchanged in the case of HBeAg, while a markedly larger percentage of positives (7% higher) was detected for anti-HBe using the monoclonal antibodies.

摘要

对两种用于检测与乙型肝炎病毒感染相关的HBeAg/抗-HBe系统的检测方法进行了比较，这两种方法分别使用了单克隆抗体（EBK - Sorin）和多克隆抗体（HBe - Abbott）。对随机抽取的人群（1000份样本）进行检测的结果表明，单克隆试剂具有更高的灵敏度，特异性没有任何损失，并且还有使用较低放射性水平的额外优势。发现两种试剂盒检测得到的HBeAg阳性和阴性比例保持不变，而使用单克隆抗体检测抗-HBe时，检测到的阳性百分比明显更高（高7%）。